Indican Assay Kit (100 tests) (BA0037)

Indican Assay Kit - Information

Assay Genie's quantitative indican assay kit is based on an improved Curzon and Walsh method. In this assay, indican reacts with a chromogen. The color intensity of the product at 480nm is directly proportional to the indican concentration in the sample.

Applications

For quantitative determination of indican (indoxyl sulfate) in urine samples.

Indican Assay Kit - Key Features

• Fast and sensitive. Linear detection range: 0.2 - 20 mg/dL (8-800 oles/L).
• Fast and high-throughput. Homogeneous "mix-incubate-measure" type assay. Assay takes only 10 min. Can be readily automated for processing thousands of samples per day.

Indican Assay Kit - Data Sheet
Kit Includes	Reagent A 20 mL, Reagent B 1.5 mL, Standard 1.5 mL 30 mg/dL
Kit Requires	For 96-well plate assays: pipetting devices, clear flat bottom 96-well plates and plate reader. For cuvette assays: pipetting devices, cuvettes and spectrophotometer (480nm).
Method of Detection	OD480nm
Detection Limit	0.2 mg/dL
Samples	Urine
Species	All
Protocol Length	10 min
Size	100 tests
Storage	Store Reagent A at room temperature and other components at -20°C
Shelf Life	12 months

More Details

Indican (indoxyl sulfate) is a putrefaction product resulting from bacterial deconjugation of dietary tryptophan to indole in the small intestine. A large body of evidence supports the use of urinary indican testing as a beneficial point-of-care screening tool for dysbiosis, small intestinal bacterial overgrowth (SIBO), constipation, malabsorption, intestinal mucosal permeability etc. Common microorganisms that contribute to positive indican tests include Salmonella, Shigella, Campylobacter jejuni, yersinia enterocolitica, Citrobacter freundi, Citrobacter diversus, Klebsiella pneumoniae, Pseudomonas aeruginosa, certain strains of Escherichia coli, Staphyloccocus aureus, some strains of bacteriodes, Clostridium difficile, Candida albicans, other candida species etc. Traditional Indican test using Obermayer's reagent gives only qualitative results.