Antibody Name:	IGLV1-51 Antibody (PACO18710)
Antibody SKU:	PACO18710
Size:	50ul
Host Species:	Rabbit
Tested Applications:	ELISA, WB, IHC
Recommended Dilutions:	ELISA:1:2000-1:5000, WB:1:500-1:2000, IHC:1:25-1:100
Species Reactivity:	Human
Immunogen:	Synthetic peptide of human Lambda Light chain

Form:	Liquid
Storage Buffer:	-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol
Purification Method:	Antigen affinity purification
Clonality:	Polyclonal
Isotype:	IgG
Conjugate:	Non-conjugated

	The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using PACO18710(Lambda Light chain Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).
	Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane: Human plasma solution, Primary antibody: PACO18710(Lambda Light chain Antibody) at dilution 1/200 dilution, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 10 seconds.
	The image on the left is immunohistochemistry of paraffin-embedded Human brain tissue using PACO18710(Lambda Light chain Antibody) at dilution 1/20, on the right is treated with synthetic peptide. (Original magnification: x200).

Background:

Antibody producing cells of the immune system require multiple rearrangements of immunoglobulin (antibody, Ig) genes. Immunoglobulins are four-chain, Y-shaped, monomeric structures of two identical heavy chains and two identical light chains held together through interchain disulfide bonds. Immunoglobulins in vertebrates help to remove non-self molecules or cells (antigens) by recognizing and binding to the antigen and carrying out effector functions that activate the immune system. Variable genetic combinations of the five heavy chain classes (M, D, G, E and A) and the two light chain isotypes, κ and Lambda, confer the role of an antibody. The variable region genes encoding immunoglobulin κ and Lambda chains are assembled from three DNA segments, the V, C and J genes. κ and Lambda consist of a variable region and a constant region and can easily be differentiated by the antigenic properties of the constant region. The ratio of κ to Lambda is 70:30 , the vast majority of which is bound to heavy-chain in immunoglobulin.

Synonyms:

Ig lambda chain V-I region NEW

UniProt Protein Function:	IGLV1-51: V segment of the variable region of immunoglobulins light chain that participates to the antigen recognition. Immunoglobulins (Igs), also known as antibodies, are membrane- bound or secreted glycoproteins produced by B lymphocytes. In the recognition phase of humoral immunity, the membrane-bound Igs serve as receptors which, upon binding of a specific antigen (Ag), trigger the clonal expansion and differentiation of B lymphocytes into Ig-secreting plasma cells. Secreted Igs mediate the effector phase of humoral immunity, which results in the elimination of bound antigens (PubMed:20176268, PubMed:22158414). The antigen binding site is formed by the variable region of one heavy chain, together with that of its associated light chain. Thus, each Ig has two antigen binding sites with remarkable affinity for a particular antigen. The variable regions are assembled by a process called V(D)J recombination and can then be subjected to somatic hypermutation after exposure to antigen to allow affinity maturation for a particular Ag (PubMed:20176268, PubMed:17576170). {ECO:0000303\|PubMed:17576170, ECO:0000303\|PubMed:20176268, ECO:0000303\|PubMed:22158414}. Immunoglobulins are composed of two identical heavy chains and two identical light chains, linked by disulfide bonds. {ECO:0000303\|PubMed:20176268}
UniProt Protein Details:
NCBI Summary:
UniProt Code:	P01701
NCBI GenInfo Identifier:	126541
NCBI Gene ID:	3535
NCBI Accession:	P01701.1
UniProt Secondary Accession:	P01701,P0CG04
UniProt Related Accession:	P01701
Molecular Weight:	~22.5kDa
NCBI Full Name:	Ig lambda chain V-I region NEW
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NCBI Protein Information:
UniProt Protein Name:	Ig lambda chain V-I region NEW
UniProt Synonym Protein Names:
Protein Family:
UniProt Gene Name:
UniProt Entry Name:	LV103_HUMAN