Hydrogen Peroxide Assay Kit (BA0047)

Hydrogen Peroxide Assay Kit - Information

Assay Genie's hydrogen peroxide assay kit is designed to measure peroxide concentration in biological samples without any pretreatment. The improved method utilizes the chromogenic Fe³⁺-xylenol orange reaction, in which a purple complex is formed when Fe²⁺ provided in the reagent is oxidized to Fe³⁺ by peroxides present in the sample. The intensity of the color, measured at 540-610nm, is an accurate measure of the peroxide level in the sample. The optimized formulation substantially reduces interference by substances in the raw samples.

Applications

For quantitative determination of peroxide and evaluation of drug effects on hydrogen peroxide metabolism.

Hydrogen Peroxide Assay Kit - Key Features

• Sensitive and accurate. Enhanced color intensity using sorbitol. Detection range 0.2 (7 ng/mL) to 30 (1,020 ng/mL) hydrogen peroxidein 96-well plate assay.
• Simple and high-throughput. The procedure involves addition of a single detection reagent and incubation for 30 min. Can be readily automated as a high-throughput assay in 96-well plates for thousands of samples per day.

Hydrogen Peroxide Assay Kit - Data Sheet
Kit Includes	Reagent A: 1 mL Reagent B: 50 mL Standard: 100 mL 3% stabilized H2O2.
Kit Requires	Pipetting devices and accessories, 96-well plates and plate reader.
Method of Detection	OD585nm
Detection Limit	0.2
Samples	Serum, citrate-plasma, urine, cell lysate, culture media etc
Species	All
Protocol Length	30 min
Size	250 tests
Storage	Store all reagents at 4 °C
Shelf Life	6 months

More Details

Peroxide (e.g. hydrogen peroxide H₂O₂) is one of the key reactive oxygen species formed under oxidative stress conditions. High levels of peroxide formation have been linked to pathological conditions such as ageing, asthma, diabetes, atherosclerosis, cataract, inflammatory arthritis and neurodegenerative diseases. Simple, direct and automation-ready procedures for quantitative determination of peroxide find wide applications in research and drug discovery.