Human Cell Biology ELISA Kits 1
HumanWISP1 (WNT1 Inducible Signaling Pathway Protein 1) ELISA Kit (HUES03519)
- SKU:
- HUES03519
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- O95388
- Sensitivity:
- 46.88pg/mL
- Range:
- 78.13-5000pg/mL
- ELISA Type:
- Sandwich
- Reactivity:
- Human
- Sample Type:
- Serum, plasma and other biological fluids
- Research Area:
- Cell Biology
Description
Assay type: | Sandwich |
Format: | 96T |
Assay time: | 4.5h |
Reactivity: | Human |
Detection Method: | Colormetric |
Detection Range: | 78.13-5000 pg/mL |
Sensitivity: | 46.88 pg/mL |
Sample Volume Required Per Well: | 100µL |
Sample Type: | Serum, plasma and other biological fluids |
Specificity: | This kit recognizes HumanWISP1 in samples. No significant cross-reactivity or interference between HumanWISP1 and analogues was observed. |
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human WISP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human WISP1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human WISP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human WISP1. The concentration of Human WISP1 in samples can be calculated by comparing the OD of the samples to the standard curve.
UniProt Protein Function: | Downstream regulator in the Wnt/Frizzled-signaling pathway. Associated with cell survival. Attenuates p53-mediated apoptosis in response to DNA damage through activation of AKT kinase. Up-regulates the anti-apoptotic Bcl-X(L) protein. Adheres to skin and melanoma fibroblasts. In vitro binding to skin fibroblasts occurs through the proteoglycans, decorin and biglycan. |
NCBI Summary: | This gene encodes a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family. WNT1 is a member of a family of cysteine-rich, glycosylated signaling proteins that mediate diverse developmental processes. The CTGF family members are characterized by four conserved cysteine-rich domains: insulin-like growth factor-binding domain, von Willebrand factor type C module, thrombospondin domain and C-terminal cystine knot-like domain. This gene may be downstream in the WNT1 signaling pathway that is relevant to malignant transformation. It is expressed at a high level in fibroblast cells, and overexpressed in colon tumors. The encoded protein binds to decorin and biglycan, two members of a family of small leucine-rich proteoglycans present in the extracellular matrix of connective tissue, and possibly prevents the inhibitory activity of decorin and biglycan in tumor cell proliferation. It also attenuates p53-mediated apoptosis in response to DNA damage through activation of the Akt kinase. It is 83% identical to the mouse protein at the amino acid level. Multiple alternatively spliced transcript variants have been identified. [provided by RefSeq, Mar 2011] |
UniProt Code: | O95388 |
NCBI GenInfo Identifier: | 34098393 |
NCBI Gene ID: | 8840 |
NCBI Accession: | O95388. 1 |
UniProt Secondary Accession: | O95388,Q5JBS6, Q5JBS7, Q5JBS8, Q9HCS3, A8KAG6, E7EMM5 |
UniProt Related Accession: | O95388 |
Molecular Weight: | 21,550 Da |
NCBI Full Name: | WNT1-inducible-signaling pathway protein 1 |
NCBI Synonym Full Names: | WNT1 inducible signaling pathway protein 1 |
NCBI Official Symbol: | WISP1 |
NCBI Official Synonym Symbols: | CCN4; WISP1c; WISP1i; WISP1tc |
NCBI Protein Information: | WNT1-inducible-signaling pathway protein 1 |
UniProt Protein Name: | WNT1-inducible-signaling pathway protein 1 |
UniProt Synonym Protein Names: | CCN family member 4; Wnt-1-induced secreted protein |
Protein Family: | WNT1-inducible-signaling pathway protein |
UniProt Gene Name: | WISP1 |
UniProt Entry Name: | WISP1_HUMAN |
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e. g. operator, pipetting technique, washing technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data is provided below for reference only.
Concentration (pg/mL) | O.D | Average | Corrected |
5000 | 2.528 2.552 | 2.54 | 2.483 |
2500 | 1.76 1.772 | 1.766 | 1.709 |
1250 | 1.036 1.022 | 1.029 | 0.972 |
625 | 0.45 0.49 | 0.47 | 0.413 |
312.5 | 0.258 0.256 | 0.257 | 0.2 |
156.25 | 0.175 0.157 | 0.166 | 0.109 |
78.13 | 0.106 0.118 | 0.112 | 0.055 |
0 | 0.049 0.065 | 0.057 | -- |
Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level HumanWISP1 were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level HumanWISP1 were tested on 3 different plates, 20 replicates in each plate.
Intra-assay Precision | Inter-assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 20 | 20 | 20 | 20 | 20 | 20 |
Mean (pg/mL) | 227.76 | 766.45 | 2132.87 | 205.65 | 798.91 | 2247.01 |
Standard deviation | 11.48 | 37.25 | 83.18 | 10.82 | 33.23 | 68.98 |
C V (%) | 5.04 | 4.86 | 3.90 | 5.26 | 4.16 | 3.07 |
Recovery
The recovery of HumanWISP1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range (%) | Average Recovery (%) |
Serum (n=5) | 87-101 | 94 |
EDTA plasma (n=5) | 86-99 | 93 |
Cell culture media (n=5) | 93-108 | 98 |
Linearity
Samples were spiked with high concentrations of HumanWISP1 and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.
Serum (n=5) | EDTA plasma (n=5) | Cell culture media (n=5) | ||
1:2 | Range (%) | 92-103 | 94-109 | 97-109 |
Average (%) | 97 | 100 | 103 | |
1:4 | Range (%) | 87-99 | 85-97 | 85-100 |
Average (%) | 93 | 90 | 91 | |
1:8 | Range (%) | 87-98 | 87-98 | 83-93 |
Average (%) | 92 | 92 | 88 | |
1:16 | Range (%) | 92-104 | 81-96 | 86-98 |
Average (%) | 98 | 87 | 93 |
An unopened kit can be stored at 4°C for 1 month. If the kit is not used within 1 month, store the items separately according to the following conditions once the kit is received.
Item | Specifications | Storage |
Micro ELISA Plate(Dismountable) | 8 wells ×12 strips | -20°C, 6 months |
Reference Standard | 2 vials | |
Concentrated Biotinylated Detection Ab (100×) | 1 vial, 120 µL | |
Concentrated HRP Conjugate (100×) | 1 vial, 120 µL | -20°C(shading light), 6 months |
Reference Standard & Sample Diluent | 1 vial, 20 mL | 4°C, 6 months |
Biotinylated Detection Ab Diluent | 1 vial, 14 mL | |
HRP Conjugate Diluent | 1 vial, 14 mL | |
Concentrated Wash Buffer (25×) | 1 vial, 30 mL | |
Substrate Reagent | 1 vial, 10 mL | 4°C(shading light) |
Stop Solution | 1 vial, 10 mL | 4°C |
Plate Sealer | 5 pieces | |
Product Description | 1 copy | |
Certificate of Analysis | 1 copy |
- Set standard, test sample and control (zero) wells on the pre-coated plate and record theirpositions. It is recommended to measure each standard and sample in duplicate. Note: addall solutions to the bottom of the plate wells while avoiding contact with the well walls. Ensuresolutions do not foam when adding to the wells.
- Aliquot 100µl of standard solutions into the standard wells.
- Add 100µl of Sample / Standard dilution buffer into the control (zero) well.
- Add 100µl of properly diluted sample (serum, plasma, tissue homogenates and otherbiological fluids) into test sample wells.
- Cover the plate with the sealer provided in the kit and incubate for 90 min at 37°C.
- Aspirate the liquid from each well, do not wash. Immediately add 100µL of BiotinylatedDetection Ab working solution to each well. Cover the plate with a plate seal and gently mix. Incubate for 1 hour at 37°C.
- Aspirate or decant the solution from the plate and add 350µL of wash buffer to each welland incubate for 1-2 minutes at room temperature. Aspirate the solution from each well andclap the plate on absorbent filter paper to dry. Repeat this process 3 times. Note: a microplatewasher can be used in this step and other wash steps.
- Add 100µL of HRP Conjugate working solution to each well. Cover with a plate seal andincubate for 30 min at 37°C.
- Aspirate or decant the solution from each well. Repeat the wash process for five times asconducted in step 7.
- Add 90µL of Substrate Reagent to each well. Cover with a new plate seal and incubate forapproximately 15 min at 37°C. Protect the plate from light. Note: the reaction time can beshortened or extended according to the actual color change, but not by more than 30min.
- Add 50 µL of Stop Solution to each well. Note: Adding the stop solution should be done inthe same order as the substrate solution.
- Determine the optical density (OD value) of each well immediately with a microplate readerset at 450 nm.