Human ZWINT Recombinant Protein (RPPB5191)

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SKU:
RPPB5191
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O95229

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human ZWINT Recombinant Protein
Product Code:RPPB5191
Size:10µg
Species:Human
Target:ZWINT
Synonyms:ZW10 Interacting Kinetochore Protein, ZWINT, ZW10 Interactor, HZwint-1, KNTC2AP, ZWINT1, Human ZW10 Interacting Protein-1, ZW10 Interactor Kinetochore Protein zwint-1, Zwint-1, ZW10-Interacting Protein 1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ZWINT solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl, 20% glycerol and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMEAAETE AEAAALEVLA EVAGILEPVG LQEEAELPAK ILVEFVVDSQ KKDKLLCSQL QVADFLQNIL AQEDTAKGLD PLASEDTSRQ KAIAAKEQWK ELKATYREHV EAIKIGLTKA LTQMEEAQRK RTQLREAFEQ LQAKKQMAME KRRAVQNQWQ LQQEKHLQHL AEVSAEGKLL FPEAEAEAEN LPDDKPQQPT RPQEQSTGDT MGRDPGVSFK AVGLQPAGDV NLP

ZW10 Interacting Kinetochore Protein (ZWINT) is involved in kinetochore function. ZWINT is part of the MIS12 complex, which is essential for kinetochore formation and spindle checkpoint activity. ZWINT is localized to the cytoplasm during interphase and to kinetochores from late prophase to anaphase. ZWINT interacts with ZW10 (Zeste White 10) and regulates the association between ZW10 and kinetochores. ZWINT gene defects are linked with the pathogenesis of Roberts�s syndrome, an autosomal recessive disorder characterized by growth retardation due to premature chromosome separation.

ZWINT Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 253 amino acids (1-230) and having a molecular mass of 27.9 kDa (Molecular size on SDS-PAGE will appear higher).ZWINT is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ZWINT: Part of the MIS12 complex, which is required for kinetochore formation and spindle checkpoint activity. Required to target ZW10 to the kinetochore at prometaphase.
UniProt Protein Details:

Protein type:Cell cycle regulation

Chromosomal Location of Human Ortholog: 10q21-q22

Cellular Component: cytoplasm; cytosol; kinetochore

Molecular Function:protein binding; protein N-terminus binding

Biological Process: establishment of cellular localization; mitotic cell cycle checkpoint; mitotic sister chromatid segregation; sister chromatid cohesion

NCBI Summary:This gene encodes a protein that is clearly involved in kinetochore function although an exact role is not known. It interacts with ZW10, another kinetochore protein, possibly regulating the association between ZW10 and kinetochores. The encoded protein localizes to prophase kinetochores before ZW10 does and it remains detectable on the kinetochore until late anaphase. It has a uniform distribution in the cytoplasm of interphase cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:O95229
NCBI GenInfo Identifier:117949386
NCBI Gene ID:11130
NCBI Accession:O95229.2
UniProt Secondary Accession:O95229,Q0D2I3, Q9BWD0, A6NNV6,
UniProt Related Accession:O95229
Molecular Weight:25,544 Da
NCBI Full Name:ZW10 interactor
NCBI Synonym Full Names:ZW10 interacting kinetochore protein
NCBI Official Symbol:ZWINT��
NCBI Official Synonym Symbols:SIP30; ZWINT1; KNTC2AP; HZwint-1��
NCBI Protein Information:ZW10 interactor
UniProt Protein Name:ZW10 interactor
UniProt Synonym Protein Names:ZW10-interacting protein 1; Zwint-1
Protein Family:ZW10 interactor
UniProt Gene Name:ZWINT��
UniProt Entry Name:ZWINT_HUMAN
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system_update_altDatasheetHuman ZWINT Recombinant Protein ZW10 Interactor is localized to the kinetochores from late Prophase to Anaphase and has a uniform distribution in the cytoplasm of Interp