Enzymes Recombinant Proteins

Human YARS2 Recombinant Protein (RPPB2422)

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SKU:
RPPB2422
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9Y2Z4
Research Area:
Enzymes

Description

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Product Name:Human YARS2 Recombinant Protein
Product Code:RPPB2422
Size:10µg
Species:Human
Target:YARS2
Synonyms:Tyrosine--tRNA ligase mitochondrial, Tyrosyl-tRNA synthetase, TyrRS, YARS2, CGI-04, TYRRS, MLASA2, MT-TYRRS.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:YARS2 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl, 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MTLNLSVLLP LGLRKAHSGA QGLLAAQKAR GLFKDFFPET GTKIELPELF DRGTASFPQT IYCGFDPTAD SLHVGHLLAL LGLFHLQRAG HNVIALVGGA TARLGDPSGR TKEREALETE RVRANARALR LGLEALAANH QQLFTDGRSW GSFTVLDNSA WYQKQHLVDF LAAVGGHFRM GTLLSRQSVQ LRLKSPEGMS LAEFFYQVLQ AYDFYYLFQR YGCRVQLGGS DQLGNIMSGY EFINKLTGED VFGITVPLIT STTGAKLGKS AGNAVWLNRD KTSPFELYQF FVRQPDDSVE RYLKLFTFLP LPEIDHIMQL HVKEPERRGP QKRLAAEVTK LVHGREGLDS AKRCTQALYH SSIDALEVMS DQELKELFKE APFSEFFLDP GTSVLDTCRK ANAIPDGPRG YRMITEGGVS INHQQVTNPE SVLIVGQHIL KNGLSLLKIG KRNFYIIKWL QL

Tyrosyl-tRNA synthetase (YARS2) is a mitochondrial protein which catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr-AMP and afterward transferred to the acceptor end of tRNA(Tyr). YARS2 gene mutations are linked with myopathy with lactic acidosis and sideroblastic anemia type 2 (MLASA2).

YARS2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 482 amino acids (17-477 a.a.) and having a molecular mass of 53.7kDa.YARS2 is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:YARS2: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS2 are the cause of myopathy with lactic acidosis and sideroblastic anemia type 2 (MLASA2). MLASA2 is a rare oxidative phosphorylation disorder specific to skeletal muscle and bone marrow. Affected individuals manifest sideroblastic anemia, progressive lethargy, muscle weakness, and exercise intolerance associated with persistent lactic acidemia. Belongs to the class-I aminoacyl-tRNA synthetase family.
UniProt Protein Details:

Protein type:RNA-binding; Ligase; Translation; Mitochondrial; EC 6.1.1.1

Chromosomal Location of Human Ortholog: 12p11.21

Cellular Component: mitochondrion; mitochondrial matrix

Molecular Function:protein homodimerization activity; tyrosine-tRNA ligase activity; ATP binding; tRNA binding

Biological Process: tRNA aminoacylation for protein translation; translation; gene expression; tRNA aminoacylation

Disease: Myopathy, Lactic Acidosis, And Sideroblastic Anemia 2

NCBI Summary:This gene encodes a mitochondrial protein that catalyzes the attachment of tyrosine to tRNA(Tyr). Mutations in this gene are associated with myopathy with lactic acidosis and sideroblastic anemia type 2 (MLASA2). [provided by RefSeq, Jan 2011]
UniProt Code:Q9Y2Z4
NCBI GenInfo Identifier:50401709
NCBI Gene ID:51067
NCBI Accession:Q9Y2Z4.2
UniProt Secondary Accession:Q9Y2Z4,Q9H817, D3DUW8,
UniProt Related Accession:Q9Y2Z4
Molecular Weight:477
NCBI Full Name:Tyrosine--tRNA ligase, mitochondrial
NCBI Synonym Full Names:tyrosyl-tRNA synthetase 2, mitochondrial
NCBI Official Symbol:YARS2��
NCBI Official Synonym Symbols:TYRRS; CGI-04; MLASA2; MT-TYRRS��
NCBI Protein Information:tyrosine--tRNA ligase, mitochondrial; tyrosine tRNA ligase 2, mitochondrial
UniProt Protein Name:Tyrosine--tRNA ligase, mitochondrial
UniProt Synonym Protein Names:Tyrosyl-tRNA synthetase; TyrRS
UniProt Gene Name:YARS2��
UniProt Entry Name:SYYM_HUMAN
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