Enzymes Recombinant Proteins

Human YARS Recombinant Protein (RPPB2421)

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RPPB2421

Description

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Product Name:Human YARS Recombinant Protein
Product Code:RPPB2421
Size:10µg
Species:Human
Target:YARS
Synonyms:Tyrosyl-tRNA synthetase cytoplasmic, Tyrosyl--tRNA ligase, TyrRS, YARS, YRS, YTS, CMTDIC.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:YARS protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGDAPSPEEK LHLITRNLQE VLGEEKLKEI LKERELKIYW GTATTGKPHV AYFVPMSKIA DFLKAGCEVT ILFADLHAYL DNMKAPWELL ELRVSYYENV IKAMLESIGV PLEKLKFIKG TDYQLSKEYT LDVYRLSSVV TQHDSKKAGA EVVKQVEHPL LSGLLYPGLQ ALDEEYLKVD AQFGGIDQRK IFTFAEKYLP ALGYSKRVHL MNPMVPGLTG SKMSSSEEES KIDLLDRKED VKKKLKKAFC EPGNVENNGV LSFIKHVLFP LKSEFVILRD EKWGGNKTYT AYVDLEKDFA AEVVHPGDLK NSVEVALNKL LDPIREKFNT PALKKLASAA YPDPSKQKPM AKGPAKNSEP EEVIPSRLDI RVGKIITVEK HPDADSLYVE KIDVGEAEPR TVVSGLVQFV PKEELQDRLV VVLCNLKPQK MRGVESQGML LCASIEGINR QVEPLDPPAG SAPGEHVFVK GYEKGQPDEE LKPKKKVFEK LQADFKISEE CIAQWKQTNF MTKLGSISCK SLKGGNIS

Tyrosyl-tRNA synthetase (YARS) is a member of the class I tRNA synthetase family. YARS is a vital enzyme which catalyzes the aminoacylation of tRNATrp with tryptophan, a critical function of the cell�s protein synthesis machinery. Expression of YARS is highly stimulated in human cells by the addition of IFN-gamma.

YARS produced in E.Coli is a single, non-glycosylated polypeptide chain containing 548 amino acids (1-528 a.a.) and having a molecular mass of 61.3kDa.YARS is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:YARS: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction: tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr). Defects in YARS are the cause of Charcot-Marie-Tooth disease dominant intermediate type C (CMTDIC). CMTDIC is a form of Charcot-Marie-Tooth disease characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Belongs to the class-I aminoacyl-tRNA synthetase family.
UniProt Protein Details:

Protein type:EC 6.1.1.1; Ligase

Chromosomal Location of Human Ortholog: 1p35.1

Cellular Component: cytoplasm; cytosol; extracellular space; nucleus

Molecular Function:interleukin-8 receptor binding; protein binding; tyrosine-tRNA ligase activity; valine-tRNA ligase activity

Biological Process: apoptosis; tRNA aminoacylation for protein translation; valyl-tRNA aminoacylation

Disease: Charcot-marie-tooth Disease, Dominant Intermediate C

NCBI Summary:Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Tyrosyl-tRNA synthetase belongs to the class I tRNA synthetase family. Cytokine activities have also been observed for the human tyrosyl-tRNA synthetase, after it is split into two parts, an N-terminal fragment that harbors the catalytic site and a C-terminal fragment found only in the mammalian enzyme. The N-terminal fragment is an interleukin-8-like cytokine, whereas the released C-terminal fragment is an EMAP II-like cytokine. [provided by RefSeq, Jul 2008]
UniProt Code:P54577
NCBI GenInfo Identifier:13638438
NCBI Gene ID:8565
NCBI Accession:P54577.4
UniProt Secondary Accession:P54577,O43276, Q53EN1, B3KWK4, D3DPQ4,
UniProt Related Accession:P54577
Molecular Weight:59,143 Da
NCBI Full Name:Tyrosine--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:tyrosyl-tRNA synthetase
NCBI Official Symbol:YARS��
NCBI Official Synonym Symbols:YRS; YTS; TYRRS; CMTDIC��
NCBI Protein Information:tyrosine--tRNA ligase, cytoplasmic
UniProt Protein Name:Tyrosine--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Tyrosyl-tRNA synthetase; TyrRS
Protein Family:Tyrosine--tRNA ligase
UniProt Gene Name:YARS��
UniProt Entry Name:SYYC_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P54577
Research Area:
Enzymes
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