Human WDR5 Recombinant Protein (RPPB5933)

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RPPB5933

Description

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Product Name:Human WDR5 Recombinant Protein
Product Code:RPPB5933
Size:20µg
Species:Human
Target:WDR5
Synonyms:WDR5, BIG-3, SWD3, BIG3, WD Repeat Domain 5, WD repeat-containing protein 5, BMP2-induced 3-kb gene protein.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:The WDR5 protein solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MATEEKKPET EAARAQPTPS SSATQSKPTP VKPNYALKFT LAGHTKAVSS VKFSPNGEWL ASSSADKLIK IWGAYDGKFE KTISGHKLGI SDVAWSSDSN LLVSASDDKT LKIWDVSSGK CLKTLKGHSN YVFCCNFNPQ SNLIVSGSFD ESVRIWDVKT GKCLKTLPAH SDPVSAVHFN RDGSLIVSSS YDGLCRIWDT ASGQCLKTLI DDDNPPVSFV KFSPNGKYIL AATLDNTLKL WDYSKGKCLK TYTGHKNEKY CIFANFSVTG GKWIVSGSED NLVYIWNLQT KEIVQKLQGH TDVVISTACH PTENIIASAA LENDKTIKLW KSDC

WDR5, also designated BMP-2-induced gene 3 kb or BIG-3, belongs to the family of WD-40 repeat proteins, and is essential for vertebrate development, Hox gene activation and global H3K4 trimethylation. This protein is expressed in osteoblasts, chondrocytes, osteocytes and marrow stromal cells. WDR5 may play a role in its function of accelerating osteoblast differentiation.

WDR5 Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 354 amino acids (1-334 a.a.) and having a molecular mass of 38.8 kDa. The WDR5 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:WDR5: a WD40 repeat protein. May accelerate osteoblast differentiation. Interacts with HCFC1. WD40 repeats are found in a number of eukaryotic proteins that coordinate multi-protein complex assemblies. WD40 proteins are implicated in many functions including adaptor/regulatory modules in signal transduction, pre-mRNA processing and cytoskeleton assembly.
UniProt Protein Details:

Protein type:Adaptor/scaffold

Chromosomal Location of Human Ortholog: 9q34

Cellular Component: histone acetyltransferase complex; histone methyltransferase complex; nucleoplasm; nucleus

Molecular Function:histone acetyltransferase activity (H4-K16 specific); histone lysine N-methyltransferase activity (H3-K4 specific); histone-lysine N-methyltransferase activity; methylated histone residue binding; protein binding

Biological Process: histone H3-K4 methylation

NCBI Summary:This gene encodes a member of the WD repeat protein family. WD repeats are minimally conserved regions of approximately 40 amino acids typically bracketed by gly-his and trp-asp (GH-WD), which may facilitate formation of heterotrimeric or multiprotein complexes. Members of this family are involved in a variety of cellular processes, including cell cycle progression, signal transduction, apoptosis, and gene regulation. This protein contains 7 WD repeats. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Jul 2008]
UniProt Code:P61964
NCBI GenInfo Identifier:48429182
NCBI Gene ID:11091
NCBI Accession:P61964.1
UniProt Secondary Accession:P61964,Q91VA5, Q9NWX7, Q9UGP9,
UniProt Related Accession:P61964
Molecular Weight:36,588 Da
NCBI Full Name:WD repeat-containing protein 5
NCBI Synonym Full Names:WD repeat domain 5
NCBI Official Symbol:WDR5��
NCBI Official Synonym Symbols:SWD3; BIG-3; CFAP89��
NCBI Protein Information:WD repeat-containing protein 5
UniProt Protein Name:WD repeat-containing protein 5
UniProt Synonym Protein Names:BMP2-induced 3-kb gene protein
Protein Family:WD repeat-containing protein
UniProt Gene Name:WDR5��
UniProt Entry Name:WDR5_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P61964
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