Enzymes Recombinant Proteins

Human WARS Recombinant Protein (RPPB2417)

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SKU:
RPPB2417
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P23381
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human WARS Recombinant Protein
Product Code:RPPB2417
Size:25µg
Species:Human
Target:WARS
Synonyms:GAMMA-2, IFI53, IFP53, WRS, WARS, TrpRS, hWRS, EC=6.1.1.2, Tryptophanyl-tRNA synthetase, INF-induced protein 53, Tryptophan--tRNA ligase, GAMMA-2.
Source:Escherichia Coli
Physical Appearance:Sterile filtered colorless solution.
Formulation:1mg/ml solution containing 20mM Tris-HCl pH-8, 1mM DTT, 0.1M NaCl, 1mM DTT & 10% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MPNSEPASLL ELFNSIATQG ELVRSLKAGN ASKDEIDSAV KMLVSLKMSY KAAAGEDYKA DCPPGNPAPT SNHGPDATEA EEDFVDPWTV QTSSAKGIDY DKLIVRFGSS KIDKELINRI ERATGQRPHH FLRRGIFFSH RDMNQVLDAY ENKKPFYLYT GRGPSSEAMH VGHLIPFIFT KWLQDVFNVP LVIQMTDDEK YLWKDLTLDQ AYSYAVENAK DIIACGFDIN KTFIFSDLDY MGMSSGFYKN VVKIQKHVTF NQVKGIFGFT DSDCIGKISF PAIQAAPSFS NSFPQIFRDR TDIQCLIPCA IDQDPYFRMT RDVAPRIGYP KPALLHSTFF PALQGAQTKM SASDPNSSIF LTDTAKQIKT KVNKHAFSGG RDTIEEHRQF GGNCDVDVSF MYLTFFLEDD DKLEQIRKDY TSGAMLTGEL KKALIEVLQP LIAEHQARRK EVTDEIVKEF MTPRKLSFDF Q

WARS is part of the class I tRNA synthetase family. 2 types of tryptophanyl tRNA synthetase exist, a cytoplasmic form, called WARS, and a mitochondrial form, called WARS2. WARS catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by INF. WARS controls ERK, Akt, and eNOS activation pathways that are related with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression.

WARS Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 491 amino acids (1-471 a.a.) and having a molecular mass of 55.3 kDa. The WARS is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.

UniProt Protein Function:WARS: Isoform 1, isoform 2 and T1-TrpRS have aminoacylation activity while T2-TrpRS lacks it. Isoform 2, T1-TrpRS and T2-TrpRS possess angiostatic activity whereas isoform 1 lacks it. T2-TrpRS inhibits fluid shear stress-activated responses of endothelial cells. Regulates ERK, Akt, and eNOS activation pathways that are associated with angiogenesis, cytoskeletal reorganization and shear stress-responsive gene expression. Belongs to the class-I aminoacyl-tRNA synthetase family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Translation; Amino Acid Metabolism - tryptophan; Ligase; EC 6.1.1.2

Chromosomal Location of Human Ortholog: 14q32.31

Cellular Component: cytoplasm; nucleus; cytosol

Molecular Function:protein binding; tryptophan-tRNA ligase activity; ATP binding

Biological Process: negative regulation of cell proliferation; tRNA aminoacylation for protein translation; tryptophanyl-tRNA aminoacylation; translation; gene expression; angiogenesis; regulation of angiogenesis

NCBI Summary:Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAs, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of tryptophanyl-tRNA synthetase exist, a cytoplasmic form, named WARS, and a mitochondrial form, named WARS2. Tryptophanyl-tRNA synthetase (WARS) catalyzes the aminoacylation of tRNA(trp) with tryptophan and is induced by interferon. Tryptophanyl-tRNA synthetase belongs to the class I tRNA synthetase family. Four transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:P23381
NCBI GenInfo Identifier:135191
NCBI Gene ID:7453
NCBI Accession:P23381.2
UniProt Secondary Accession:P23381,P78535, Q502Y0, Q53XB6, Q9UDI5, Q9UDL3, A6NGN1 A6NID3,
UniProt Related Accession:P23381
Molecular Weight:471
NCBI Full Name:Tryptophan--tRNA ligase, cytoplasmic
NCBI Synonym Full Names:tryptophanyl-tRNA synthetase
NCBI Official Symbol:WARS��
NCBI Official Synonym Symbols:IFI53; IFP53; GAMMA-2��
NCBI Protein Information:tryptophan--tRNA ligase, cytoplasmic; hWRS; trpRS; interferon-induced protein 53; tryptophan tRNA ligase 1, cytoplasmic
UniProt Protein Name:Tryptophan--tRNA ligase, cytoplasmic
UniProt Synonym Protein Names:Interferon-induced protein 53; IFP53; Tryptophanyl-tRNA synthetase; TrpRS; hWRS
Protein Family:Tryptophan--tRNA ligase
UniProt Gene Name:WARS��
UniProt Entry Name:SYWC_HUMAN
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system_update_altDatasheetHuman WARS/TrpRS Recombinant Protein There exists two types of tryptophanyl tRNA synthetases, the cytoplasmic form called WARS, the mitochondrial form called WARS2. WARS