Human uPA / Urokinase-Type Plasminogen Activator ELISA Kit
- SKU:
- HUFI00278
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P00749
- Sensitivity:
- 37.5pg/ml
- Range:
- 62.5-4000pg/ml
- ELISA Type:
- Sandwich
- Synonyms:
- uPA, PLAU, Urokinase, ATF, plasminogen activator, urokinase, UPA, u-PA, U-plasminogen activator, urinary, urokinase-type plasminogen activator
- Reactivity:
- Human
Description
| Product Name: | Human uPA / Urokinase-Type Plasminogen Activator ELISA Kit |
| Product Code: | HUFI00278 |
| Size: | 96 Assays |
| Alias: | uPA, PLAU, Urokinase, ATF, plasminogen activator, urokinase, UPA, u-PA, U-plasminogen activator, urinary, urokinase-type plasminogen activator |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human PLAU/uPA concentrations in serum plasma and other biological fluids. |
| Sensitivity: | 37.5pg/ml |
| Range: | 62.5-4000pg/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human PLAU/uPA and the recovery rates were calculated by comparing the measured value to the expected amount of Human PLAU/uPA in samples. | ||||||||||||||||
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| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human PLAU/uPA and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. | ||||||||||||||||
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| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| Uniprot | P00749 |
| UniProt Protein Function: | Function: Specifically cleaves the zymogen plasminogen to form the active enzyme plasmin. |
| UniProt Protein Details: | Catalytic activity: Specific cleavage of Arg--Val bond in plasminogen to form plasmin. Enzyme regulation: Inhibited by SERPINA5. Ref.17 Ref.22 Subunit structure: Found in high and low molecular mass forms. Each consists of two chains, A and B. The high mol Fill out our quote form below and a dedicated member of staff will get back to you within one working day! Human Cardiovascular ELISA Kits |
