Human ULBP2 Recombinant Protein (RPPB5081)

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RPPB5081

Description

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Product Name:Human ULBP2 Recombinant Protein
Product Code:RPPB5081
Size:20µg
Species:Human
Target:ULBP2
Synonyms:UL16 binding protein 2, retinoic acid early transcript 1 H, NKG2D ligand 2, Retinoic acid early transcript 1H, ALCAN-alpha, N2DL-2, RAET1H.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The ULBP2 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2M urea, 0.2M NaCl, 2mM DTT and 30% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 85% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMGRADP HSLCYDITVI PKFRPGPRWC AVQGQVDEKT FLHYDCGNKT VTPVSPLGKK LNVTTAWKAQ NPVLREVVDI LTEQLRDIQL ENYTPKEPLT LQARMSCEQK AEGHSSGSWQ FSFDGQIFLL FDSEKRMWTT VHPGARKMKE KWENDKVVAM SFHYFSMGDC IGWLEDFLMG MDSTLEPSAG APLAMS

ULBP2 is a member of the MHC class I family. ULBP2 is ligand for the NKG2D receptor, composed with at least ULBP1 and ULBP3. ULBPs promote multiple signaling pathways in primary NK cells, triggering the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D encourages calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, ULBP2 cooperates with soluble CMV glycoprotein UL16. This cooperation is blocked with the NKG2D receptor, providing a mechanism in which CMV infected cells can escape the immune system. Additionally, UL16 causes ULBP2 to be held in the ER and cis-Golgi apparatus so that it does not reach the cell surface.

ULBP2 Human Recombinant produced in E. coli is a single polypeptide chain containing 216 amino acids (26-216) and having a molecular mass of 24.3 kDa.ULBP2 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ULBP2: Ligand for the NKG2D receptor, together with at least ULBP1 and ULBP3. ULBPs activate multiple signaling pathways in primary NK cells, resulting in the production of cytokines and chemokines. Binding of ULBPs ligands to NKG2D induces calcium mobilization and activation of the JAK2, STAT5, ERK and PI3K kinase/Akt signal transduction pathway. In CMV infected cells, interacts with soluble CMV glycoprotein UL16. The interaction with UL16 blocked the interaction with the NKG2D receptor, providing a mechanism by which CMV infected cells might escape the immune system. UL16 also causes ULBP2 to be retained in the ER and cis- Golgi apparatus so that it does not reach the cell surface. Belongs to the MHC class I family.
UniProt Protein Details:

Protein type:Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 6q25

Cellular Component: anchored to plasma membrane; cell surface; extracellular space

Molecular Function:antigen binding; natural killer cell lectin-like receptor binding

Biological Process: antigen processing and presentation; natural killer cell activation; natural killer cell mediated cytotoxicity

NCBI Summary:This gene encodes a major histocompatibility complex (MHC) class I-related molecule that binds to the NKG2D receptor on natural killer (NK) cells to trigger release of multiple cytokines and chemokines that in turn contribute to the recruitment and activation of NK cells. The encoded protein undergoes further processing to generate the mature protein that is either anchored to membrane via a glycosylphosphatidylinositol moiety, or secreted. Many malignant cells secrete the encoded protein to evade immunosurveillance by NK cells. This gene is located in a cluster of multiple MHC class I-related genes on chromosome 6. [provided by RefSeq, Jul 2015]
UniProt Code:Q9BZM5
NCBI GenInfo Identifier:47605923
NCBI Gene ID:80328
NCBI Accession:Q9BZM5.1
UniProt Secondary Accession:Q9BZM5,Q5VUN4,
UniProt Related Accession:Q9BZM5
Molecular Weight:27,368 Da
NCBI Full Name:NKG2D ligand 2
NCBI Synonym Full Names:UL16 binding protein 2
NCBI Official Symbol:ULBP2��
NCBI Official Synonym Symbols:N2DL2; RAET1H; NKG2DL2; ALCAN-alpha��
NCBI Protein Information:NKG2D ligand 2
UniProt Protein Name:NKG2D ligand 2
UniProt Synonym Protein Names:ALCAN-alpha; Retinoic acid early transcript 1H; UL16-binding protein 2
Protein Family:NKG2D ligand
UniProt Gene Name:ULBP2��
UniProt Entry Name:N2DL2_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9BZM5
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