Description
| Product Name: | Human UGDH Recombinant Protein |
| Product Code: | RPPB2400 |
| Size: | 10µg |
| Species: | Human |
| Target: | UGDH |
| Synonyms: | GDH, UDP-GlcDH, UDPGDH, UGD, EC=1.1.1.22, UDP-Glc dehydrogenase, UDP-glucose 6-dehydrogenase, UGDH. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | UGDH Human solution (0.5mg/ml)�containing 20mM Tris-HCl pH-8, 1mM DTT, 1mM EDTA, 0.1M NaCl & 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSELEM FEIKKICCIG AGYVGGPTCS VIAHMCPEIR VTVVDVNESR INAWNSPTLP IYEPGLKEVV ESCRGKNLFF STNIDDAIKE ADLVFISVNT PTKTYGMGKG RAADLKYIEA CARRIVQNSN GYKIVTEKST VPVRAAESIR RIFDANTKPN LNLQVLSNPE FLAEGTAIKD LKNPDRVLIG GDETPEGQRA VQALCAVYEH WVPREKILTT NTWSSELSKL AANAFLAQRI SSINSISALC EATGADVEEV ATAIGMDQRI GNKFLKASVG FGGSCFQKDV LNLVYLCEAL NLPEVARYWQ QVIDMNDYQR RRFASRIIDS LFNTVTDKKI AILGFAFKKD TGDTRESSSI YISKYLMDEG AHLHIYDPKV PREQIVVDLS HPGVSEDDQV SRLVTISKDP YEACDGAHAV VICTEWDMFK ELDYERIHKK MLKPAFIFDG RRVLDGLHNE LQTIGFQIET IGKKVSSKRI PYAPSGEIPK FSLQDPPNKK PKV |
UGDH is part of the UDP-glucose/GDP-mannose dehydrogenase family and is a widely expressed enzyme localized in the liver. UGDH transfers UDP-glucose to UDP-glucuronate and thus takes part in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated products are ordinary molecules of the extracellular matrix and participate in signal transduction, cell migration, and cancer growth and metastasis.
UGDH Recombinant Human produced in E.Coli is a single, non-glycosylated polypeptide chain containing 533 amino acids (1-494 a.a.) and having a molecular mass of 59.5 kDa. The UGDH is fused to a 39 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UGDH: Involved in the biosynthesis of glycosaminoglycans; hyaluronan, chondroitin sulfate, and heparan sulfate. Belongs to the UDP-glucose/GDP-mannose dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Oxidoreductase; Motility/polarity/chemotaxis; Carbohydrate Metabolism - starch and sucrose; EC 1.1.1.22; Carbohydrate Metabolism - ascorbate and aldarate; Carbohydrate Metabolism - amino sugar and nucleotide sugar; Carbohydrate Metabolism - pentose and glucuronate interconversions Chromosomal Location of Human Ortholog: 4p15.1 Cellular Component: nucleoplasm; cytosol Molecular Function:electron carrier activity; NAD binding; UDP-glucose 6-dehydrogenase activity Biological Process: glycosaminoglycan biosynthetic process; xenobiotic metabolic process; gastrulation with mouth forming second; UDP-glucuronate biosynthetic process; UDP-glucose metabolic process |
| NCBI Summary: | The protein encoded by this gene converts UDP-glucose to UDP-glucuronate and thereby participates in the biosynthesis of glycosaminoglycans such as hyaluronan, chondroitin sulfate, and heparan sulfate. These glycosylated compounds are common components of the extracellular matrix and likely play roles in signal transduction, cell migration, and cancer growth and metastasis. The expression of this gene is up-regulated by transforming growth factor beta and down-regulated by hypoxia. Alternative splicing results in multiple transcript variants.[provided by RefSeq, May 2010] |
| UniProt Code: | O60701 |
| NCBI GenInfo Identifier: | 6175086 |
| NCBI Gene ID: | 7358 |
| NCBI Accession: | O60701.1 |
| UniProt Secondary Accession: | O60701,O60589, B3KUU2, B4DN25, |
| UniProt Related Accession: | O60701 |
| Molecular Weight: | 494 |
| NCBI Full Name: | UDP-glucose 6-dehydrogenase |
| NCBI Synonym Full Names: | UDP-glucose 6-dehydrogenase |
| NCBI Official Symbol: | UGDH�� |
| NCBI Official Synonym Symbols: | GDH; UGD; UDPGDH; UDP-GlcDH�� |
| NCBI Protein Information: | UDP-glucose 6-dehydrogenase; UDP-Glc dehydrogenase; UDP-glucose dehydrogenase; uridine diphospho-glucose dehydrogenase |
| UniProt Protein Name: | UDP-glucose 6-dehydrogenase |
| UniProt Gene Name: | UGDH�� |
| UniProt Entry Name: | UGDH_HUMAN |
