Enzymes Recombinant Proteins
Human UCHL3 Recombinant Protein (RPPB2395)
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- SKU:
- RPPB2395
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P15374
- Research Area:
- Enzymes
Description
| Product Name: | Human UCHL3 Recombinant Protein |
| Product Code: | RPPB2395 |
| Size: | 20µg |
| Species: | Human |
| Target: | UCHL3 |
| Synonyms: | Ubiquitin Carboxyl-Terminal Esterase L3 (ubiquitin thiolesterase), UCH-L3, Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3, EC 3.4.19.12. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The UCHL3 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0)1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Please avoid freeze thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MEGQRWLPLE ANPEVTNQFL KQLGLHPNWQ FVDVYGMDPE LLSMVPRPVC AVLLLFPITE KYEVFRTEEE EKIKSQGQDV TSSVYFMKQT ISNACGTIGL IHAIANNKDK MHFESGSTLK KFLEESVSMS PEERARYLEN YDAIRVTHET SAHEGQTEAP SIDEKVDLHF IALVHVDGHL YELDGRKPFP INHGETSDET LLEDAIEVCK KFMERDPDEL RFNAIALSAA |
| Biological Activity: | Specific activity: >3,000 pmole/min/ug. Measured by the hydrolysis of Ubiquitin-AMC at pH 8.0, at 37C. |
Ubiquitin carboxyl-terminal hydrolase isozyme L3 belongs to a gene family whose products hydrolyze small C-terminal adducts of ubiquitin to produce the ubiquitin monomer. UCHL3 takes part in the regulation of neuronal development and spermatogenesis and is associated to neurodegenerative diseases. UCHL3 has a 54% homology to UCHL1.
UCHL3 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 250 amino acids (1-230a.a.) and having a molecular mass of 28.3kDa.UCHL3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | UCHL3: Deubiquitinating enzyme (DUB) that controls levels of cellular ubiquitin through processing of ubiquitin precursors and ubiquitinated proteins. Thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. Has a 10-fold preference for Arg and Lys at position P3. Deubiquitinates ENAC in apical compartments, thereby regulating apical membrane recycling. Indirectly increases the phosphorylation of IGFIR, AKT and FOXO1 and promotes insulin- signaling and insulin-induced adipogenesis. Required for stress- response retinal, skeletal muscle and germ cell maintenance. May be involved in working memory. Can hydrolyze UBB(+1), a mutated form of ubiquitin which is not effectively degraded by the proteasome and is associated with neurogenerative disorders. Preferentially binds diubiquitin; the interaction does not hydrolyze diubiquitin but, in vitro, inhibits the hydrolyzing activity on other substrates. Highly expressed in heart, skeletal muscle, and testis. Inhibited by monoubiquitin and diubiquitin. Belongs to the peptidase C12 family. |
| UniProt Protein Details: | Protein type:EC 3.4.19.12; Ubiquitin conjugating system; Protease Chromosomal Location of Human Ortholog: 13q22.2 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:peptidase activity; ubiquitin binding; protein binding; ubiquitin-specific protease activity Biological Process: ubiquitin-dependent protein catabolic process; protein catabolic process |
| NCBI Summary: | The protein encoded by this gene is a member of the deubiquitinating enzyme family. Members of this family are proteases that catalyze the removal of ubiquitin from polypeptides and are divided into five classes, depending on the mechanism of catalysis. This protein may hydrolyze the ubiquitinyl-N-epsilon amide bond of ubiquitinated proteins to regenerate ubiquitin for another catalytic cycle. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012] |
| UniProt Code: | P15374 |
| NCBI GenInfo Identifier: | 5174741 |
| NCBI Gene ID: | 7347 |
| NCBI Accession: | NP_005993.1 |
| UniProt Secondary Accession: | P15374,Q5TBK8, Q6IBE9, B2R970, |
| UniProt Related Accession: | P15374 |
| Molecular Weight: | 26,183 Da |
| NCBI Full Name: | ubiquitin carboxyl-terminal hydrolase isozyme L3 |
| NCBI Synonym Full Names: | ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) |
| NCBI Official Symbol: | UCHL3�� |
| NCBI Official Synonym Symbols: | UCH-L3�� |
| NCBI Protein Information: | ubiquitin carboxyl-terminal hydrolase isozyme L3; OTTHUMP00000018509; ubiquitin thiolesterase; ubiquitin thioesterase L3 |
| UniProt Protein Name: | Ubiquitin carboxyl-terminal hydrolase isozyme L3 |
| UniProt Synonym Protein Names: | Ubiquitin thioesterase L3 |
| Protein Family: | Ubiquitin carboxyl-terminal hydrolase |
| UniProt Gene Name: | UCHL3�� |
| UniProt Entry Name: | UCHL3_HUMAN |
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