Enzymes Recombinant Proteins

Human UBE2I His Recombinant Protein (RPPB2380)

(No reviews yet) Write a Review
SKU:
RPPB2380
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P63279
Research Area:
Enzymes

Description

system_update_altDatasheetsystem_update_altMSDS
Product Name:Human UBE2I His Recombinant Protein
Product Code:RPPB2380
Size:50µg
Species:Human
Target:UBE2I His
Synonyms:SUMO-conjugating enzyme UBC9, EC 6.3.2.-, SUMO-protein ligase, Ubiquitin-conjugating enzyme E2 I, Ubiquitin-protein ligase I, Ubiquitin carrier protein I, Ubiquitin carrier protein 9, p18, UBC9, C358B7.1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered white lyophilized powder.
Formulation:Lyophilized from a 0.2?m filtered concentrated (1mg/ml) solution in 1X PBS and 1mM DTT, pH 7.5.
Solubility:It is recommended to reconstitute the lyophilized UBE2I in sterile water not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized UBE2I although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution UBE2I should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:MHHHHHHAMGTLNMSGIALSRLAQERKAWRKDHPFGFVAVPTKNPDGTMNLMNWECAIPGKKGTPWEGGLFKLRMLFKDDYPSSPPKCKFEPPLFHPNVYPSGTVCLSILEEDKDWRPAITIKQILLGIQELLNEPNIQDPAQAEAYTIYCQNRVEYEKRVRAQAKKFAPS

Human Ubquitin Conjugating Enzyme 9 (Ubc9) is a member of the E2 family and is specific for the conjugation of SUMO to a variety of target proteins. SUMO conjugation to target proteins is mediated by a different, but analogous, pathway to ubiquitinylation. This E2 is unusual in that it interacts directly with protein substrates that are modified by sumolyation, and may play a role in substrate recognition. Ubc9 can mediate the conjugation of SUMO-1 to a variety of proteins including RanGAP1 and PML without the requirement of an E3 ligase.

Ubiquitin-Conjugating Enzyme E2I Human Recombinant produced in E.coli is a 19.5 kDa protein containing 171 amino acids.The UBE2I protein contains 6xHis tag and is purified by proprietary chromatographic techniques.

UniProt Protein Function:Function: E2 ubiquitin-like--protein ligase mediating SUMO/Smt3 attachment to septins and PCNA. Seems to be involved in degradation of S- (CLB5) and M-phase cyclins (CLB2). Ref.4 Ref.5
UniProt Protein Details:

Catalytic activity: ATP + SUMO + protein lysine = AMP + diphosphate + protein N-SUMOyllysine.

Pathway: Protein modification; protein sumoylation.

Subunit structure: Interacts with SIZ1. Ref.5 Ref.6

Subcellular location: Nucleus Ref.7.

Miscellaneous: Present with 2600 molecules/cell in log phase SD medium. Ref.8

Sequence similarities: Belongs to the ubiquitin-conjugating enzyme family.

UniProt Code:P63279
NCBI GenInfo Identifier:
NCBI Gene ID:
NCBI Accession:
Molecular Weight:17,911 Da
NCBI Full Name:UBC9
UniProt Protein Name:SUMO-conjugating enzyme UBC9
UniProt Synonym Protein Names:Ubiquitin carrier protein 9; Ubiquitin-conjugating enzyme E2-18 kDa; Ubiquitin-protein ligase
UniProt Gene Name:UBC9��
UniProt Entry Name:UBC9_YEAST
View AllClose

0 Reviews

View AllClose

Related Products

Human UBE2I/UBC9 Recombinant Protein (RPES1628)

Human UBE2I/UBC9 Recombinant Protein (RPES1628)

Cell Cycle Recombinant Proteins

system_update_altDatasheetHuman UBE2I/UBC9 Recombinant Protein SUMO-Conjugating Enzyme UBC9 (UBC9) belongs to the ubiquitin-conjugating enzyme family. UBC9 is homologous to ubiquitin-conjugating