Enzymes Recombinant Proteins

Human UBE2E1 Recombinant Protein (RPPB2373)

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SKU:
RPPB2373
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P51965
Research Area:
Enzymes

Description

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Product Name:Human UBE2E1 Recombinant Protein
Product Code:RPPB2373
Size:20µg
Species:Human
Target:UBE2E1
Synonyms:Ubiquitin carrier protein E1, Ubiquitin-conjugating enzyme E2E 1 (homologous to yeast UBC4/5), Ubiquitin-protein ligase E1, UBCH6, EC 6.3.2.19.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The UBE2E1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.15M NaCl and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSMSDDDSR ASTSSSSSSS SNQQTEKETN TPKKKESKVS MSKNSKLLST SAKRIQKELA DITLDPPPNC SAGPKGDNIY EWRSTILGPP GSVYEGGVFF LDITFTPEYP FKPPKVTFRT RIYHCNINSQ GVICLDILKD NWSPALTISK VLLSICSLLT DCNPADPLVG SIATQYMTNR AEHDRMARQW TKRYAT

UBE2E1 belongs to the E2 ubiquitin-conjugating enzyme family. UBE2E1 accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. UBE2E1 catalyzes the covalent attachment of ISG15 to other proteins. UBE2E1 mediates the selective degradation of short-lived and abnormal proteins. In vitro, UBE2E1 also catalyzes 'Lys-48'-linked polyubiquitination.

UBE2E1 Human Recombinant produced in E. coli is a single polypeptide chain containing 216 amino acids (1-193) and having a molecular mass of 23.8 kDa.UBE2E1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination.
NCBI Summary:The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. Three alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jan 2011]
UniProt Code:P51965
NCBI GenInfo Identifier:4507779
NCBI Gene ID:7324
NCBI Accession:NP_003332.1
UniProt Secondary Accession:P51965,B2RBX4, C9J8K2, K4DI90,
UniProt Related Accession:P51965
Molecular Weight:19,499 Da
NCBI Full Name:ubiquitin-conjugating enzyme E2 E1 isoform 1
NCBI Synonym Full Names:ubiquitin conjugating enzyme E2 E1
NCBI Official Symbol:UBE2E1��
NCBI Official Synonym Symbols:UBCH6��
NCBI Protein Information:ubiquitin-conjugating enzyme E2 E1
UniProt Protein Name:Ubiquitin-conjugating enzyme E2 E1
UniProt Synonym Protein Names:(E3-independent) E2 ubiquitin-conjugating enzyme E1 (EC:2.3.2.24
Protein Family:Ubiquitin-conjugating enzyme
UniProt Gene Name:UBE2E1��
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