Human TWF1 Recombinant Protein (RPPB5043)
- SKU:
- RPPB5043
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q12792
Description
| Product Name: | Human TWF1 Recombinant Protein |
| Product Code: | RPPB5043 |
| Size: | 10µg |
| Species: | Human |
| Target: | TWF1 |
| Synonyms: | Twinfilin-1, Protein A6, Protein tyrosine kinase 9, TWF1, PTK9. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | TWF1 protein solution (0.25mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 40% glycerol, 0.15M NaCl and 1mM DTT. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMMLYAA TRATLKKEFG GGHIKDEVFG TVKEDVSLHG YKKYLLSQSS PAPLTAAEEE LRQIKINEVQ TDVGVDTKHQ TLQGVAFPIS REAFQALEKL NNRQLNYVQL EIDIKNEIII LANTTNTELK DLPKRIPKDS ARYHFFLYKH SHEGDYLESI VFIYSMPGYT CSIRERMLYS SCKSRLLEIV ERQLQMDVIR KIEIDNGDEL TADFLYEEVH PKQHAHKQSF AKPKGPAGKR GIRRLIRGPA ETEATTD |
Twinfilin (TWF1) is a ubiquitous actin-monomer-binding protein which is composed of two ADF-homology domains. Twinfilin forms a 1:1 complex with ADP-actin-monomers, prevents nucleotide exchange on actin monomers and blocks assembly of the monomer into filaments. TWF1 is composed of 2 ADF/cofilin-like (ADF-H) domains joined by a short linker region and followed by a C-terminal tail of approximately 20 residues. The 2 ADF-H domains are approximately 20% homologous to ADF/cofilin and to each other. TWF1 protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures might be regulated by the small GTPase RAC1.
TWF1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 277 amino acids (1-252 a.a.) and having a molecular mass of 31.5kDa. TWF1 is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | A6: Actin-binding protein involved in motile and morphological processes. Inhibits actin polymerization, likely by sequestering G-actin. By capping the barbed ends of filaments, it also regulates motility. Seems to play an important role in clathrin-mediated endocytosis and distribution of endocytic organelles. Interacts with G-actin; ADP-actin form and capping protein (CP). May also be able to interact with TWF2 and phosphoinositides, PI(4,5)P2. When bound to PI(4,5)P2, it is down- regulated. Expressed at high levels in the colon, testis, ovary, prostate and lung. Expressed at lower levels in the brain, bladder and heart. Not detected in liver. Belongs to the actin-binding proteins ADF family. Twinfilin subfamily. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 12q12 Cellular Component: actin cytoskeleton; cytoplasm; filopodium; focal adhesion; intercellular junction; myofibril; perinuclear region of cytoplasm Molecular Function:actin monomer binding; ATP binding; phosphatidylinositol-4,5-bisphosphate binding; protein-tyrosine kinase activity Biological Process: negative regulation of actin filament polymerization; regulation of actin phosphorylation; sequestering of actin monomers |
| NCBI Summary: | This gene encodes twinfilin, an actin monomer-binding protein conserved from yeast to mammals. Studies of the mouse counterpart suggest that this protein may be an actin monomer-binding protein, and its localization to cortical G-actin-rich structures may be regulated by the small GTPase RAC1. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q12792 |
| NCBI GenInfo Identifier: | 259016376 |
| NCBI Gene ID: | 5756 |
| NCBI Accession: | Q12792.3 |
| UniProt Secondary Accession: | Q12792,Q59G07, Q5U0B1, Q6FHJ1, Q6FHL6, Q6NUK9, Q86XL6 Q8TCD3, A8K5A8, B3KXS6, B4DLX9, |
| UniProt Related Accession: | Q12792 |
| Molecular Weight: | 28,823 Da |
| NCBI Full Name: | Twinfilin-1 |
| NCBI Synonym Full Names: | twinfilin actin binding protein 1 |
| NCBI Official Symbol: | TWF1�� |
| NCBI Official Synonym Symbols: | A6; PTK9�� |
| NCBI Protein Information: | twinfilin-1 |
| UniProt Protein Name: | Twinfilin-1 |
| UniProt Synonym Protein Names: | Protein A6; Protein tyrosine kinase 9 |
| Protein Family: | Twinfilin |
| UniProt Gene Name: | TWF1�� |
| UniProt Entry Name: | TWF1_HUMAN |
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