Human TWEAK PharmaGenie ELISA Kit (SBRS1209)
- SKU:
- SBRS1209
- Product Type:
- ELISA Kit
- Reactivity:
- Human
- Applications:
- ELISA
- ELISA Type:
- Sandwich
Description
system_update_altDatasheet
Human TWEAK PharmaGenie ELISA Kit (SBRS1209)
| Product SKU: | SBRS1209 |
| Size: | 96T |
| Application: | Cross Reactivity: This ELISA kit shows no cross-reactivity with the following cytokines tested: human ANGPTL3, beta IG-H3, CA9, Cathepsin B, CD23, CHI3L1, CTLA-4, DKK-4, DPPIV, EDA-A2, Erythropoietin R, FGF-6, FGF-9, Gas 1, GITR, IGFBP-5, IL-1 F5 (FIL1 delta), IL-1 F6 (FIL1 epsilon), IL-1 F7 (FIL1 zeta), IL-1 F8 (FIL1 eta), IL-1 F9 (IL-1 H1), IL-1 F10 (IL-1HY2), IL-1 R5, IL-21 (IL-17C), IL-18, IL-20, IL-34, IL-5 R alpha, IL-10 R alpha, Layilin, Leptin R, Marapsin, Mer, MMP-7, P-Cadherin, Prostasin, PSMA (FOLH1), SIGIRR, TGF beta R3, Coagulation Factor III. |
| Uniprot: | O43508 |
| Gene ID: | 8742 / 407977 |
| Gene Names: | APO3L / DR3LG / TNFSF12 / UNQ181/PRO207 |
| Synonyms: | Tumor necrosis factor ligand superfamily member 12 (APO3 ligand) (TNF-related weak inducer of apoptosis) (TWEAK) [Cleaved into: Tumor necrosis factor ligand superfamily member 12, membrane form Tumor necrosis factor ligand superfamily member 12, secreted form] |
| Target Species: | Human |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 40 pg/ml - 3,000 pg/ml |
| Sensitivity: | 40 pg/ml |
| Recommended Dilution: | 3-20 fold |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Human TWEAK PharmaGenie ELISA Kit (SBRS1209) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
Related Products
Mouse TWEAK PharmaGenie ELISA Kit (SBRS1556)
system_update_altDatasheetMouse TWEAK PharmaGenie ELISA Kit (SBRS1556)Overview
Rat TWEAK R PharmaGenie ELISA Kit (SBRS1706)
system_update_altDatasheetRat TWEAK R PharmaGenie ELISA Kit (SBRS1706)Overview
Human TWEAK / TNFSF12 ELISA Kit
system_update_altDatasheet - तकनीकी मैनुअलsystem_update_altMSDS - तकनीक
Mouse TWEAK R PharmaGenie ELISA Kit (SBRS1557)
system_update_altDatasheetMouse TWEAK R PharmaGenie ELISA Kit (SBRS1557)Overview
Human TWEAK R / TNFRSF12 ELISA Kit
Human TWEAK R / TNFRSF12 ELISATNFRSF12A (TNF Receptor Superfamily Member 12A) is a protein-coding gene that is a minor inducer of apoptosis in certain cell types. TNFRSF12 is a receptor for...