Human TSG101 Recombinant Protein (RPPB5029)
- SKU:
- RPPB5029
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q99816
Description
| Product Name: | Human TSG101 Recombinant Protein |
| Product Code: | RPPB5029 |
| Size: | 10µg |
| Species: | Human |
| Target: | TSG101 |
| Synonyms: | TSG10, VPS23, TSG101, ESCRT-I complex subunit TSG101, Tumor susceptibility gene 101 protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile filtered colorless solution. |
| Formulation: | TSG101 protein solution (0.5mg/ml) containing 20mM Tris-HCl pH-8, 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMAVS ESQLKKMVSK YKYRDLTVRE TVNVITLYKD LKPVLDSYVF NDGSSRELMN LTGTIPVPYR GNTYNIPICL WLLDTYPYNP PICFVKPTSS MTIKTGKHVD ANGKIYLPYL HEWKHPQSDL LGLIQVMIVV FGDEPPVFSR P |
TSG101 is a member of apparently inactive homologs of ubiquitin-conjugating enzymes. TSG101 contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. TSG101 is involved in cell growth and differentiation and acts as a negative growth regulator. TSG101 in vitro steady-state expression is important for maintenance of genomic stability and cell cycle regulation. TSG101 mutations and alternative splicing occur in high rate in breast cancer and implicate that defects occur during breast cancer tumorigenesis and/or progression. TSG101 is a factor of the ESCRT-I complex, a monitor of vesicular trafficking process. TSG101 binds to ubiquitinated cargo proteins and is needed for the sorting of endocytic ubiquitinated cargos into multivesicular bodies. TSG101 is needed for completion of cytokinesis and is involved in cell growth and differentiation.
TSG101 Human Recombinant produced in E.Coli is a single, non-glycosylated,polypeptide chain containing 181 amino acids (1-145 a.a.) and having a molecular mass of 20.7 kDa. TSG101 protein is fused to a 36 amino acid His-Tag at N-terminus and purified by standard chromatography.
| UniProt Protein Function: | TSG101: Component of the ESCRT-I complex, a regulator of vesicular trafficking process. Binds to ubiquitinated cargo proteins and is required for the sorting of endocytic ubiquitinated cargos into multivesicular bodies (MVBs). Mediates the association between the ESCRT-0 and ESCRT-I complex. Required for completion of cytokinesis; the function requires CEP55. May be involved in cell growth and differentiation. Acts as a negative growth regulator. Involved in the budding of many viruses through an interaction with viral proteins that contain a late-budding motif P-[ST]-A-P. This interaction is essential for viral particle budding of numerous retroviruses. Belongs to the ubiquitin-conjugating enzyme family. UEV subfamily. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Nuclear receptor co-regulator; Cell cycle regulation Chromosomal Location of Human Ortholog: 11p15 Cellular Component: multivesicular body; late endosome membrane; early endosome; cytoplasm; late endosome; plasma membrane; nucleolus; endosome membrane Molecular Function:ubiquitin binding; protein binding; protein homodimerization activity; DNA binding; ubiquitin protein ligase binding; transcription corepressor activity; calcium-dependent protein binding Biological Process: viral reproduction; ubiquitin-dependent protein catabolic process via the multivesicular body pathway; non-lytic virus budding; protein modification process; viral infectious cycle; virus assembly; endosome transport; keratinocyte differentiation; negative regulation of cell proliferation; protein transport; cell division; viral protein processing; regulation of cell growth; cell cycle arrest; negative regulation of transcription, DNA-dependent Disease: Breast Cancer |
| NCBI Summary: | The protein encoded by this gene belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in this gene occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q99816 |
| NCBI GenInfo Identifier: | 5454140 |
| NCBI Gene ID: | 7251 |
| NCBI Accession: | NP_006283.1 |
| UniProt Secondary Accession: | Q99816,Q9BUM5, |
| UniProt Related Accession: | Q99816 |
| Molecular Weight: | 43,944 Da |
| NCBI Full Name: | tumor susceptibility gene 101 protein |
| NCBI Synonym Full Names: | tumor susceptibility gene 101 |
| NCBI Official Symbol: | TSG101�� |
| NCBI Official Synonym Symbols: | TSG10; VPS23�� |
| NCBI Protein Information: | tumor susceptibility gene 101 protein; tumor susceptibility protein; ESCRT-I complex subunit TSG101 |
| UniProt Protein Name: | Tumor susceptibility gene 101 protein |
| UniProt Synonym Protein Names: | ESCRT-I complex subunit TSG101 |
| Protein Family: | Tumor susceptibility gene 101 protein |
| UniProt Gene Name: | TSG101�� |
| UniProt Entry Name: | TS101_HUMAN |
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