Enzymes Recombinant Proteins

Human TSEN15 Recombinant Protein (RPPB5027)

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SKU:
RPPB5027
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q8WW01
Research Area:
Enzymes

Description

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Product Name:Human TSEN15 Recombinant Protein
Product Code:RPPB5027
Size:10µg
Species:Human
Target:TSEN15
Synonyms:tRNA-splicing endonuclease subunit Sen15, SEN15 homolog, HsSEN15, tRNA-intron endonuclease Sen15, TSEN15, C1orf19, SEN15.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The TSEN15 solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 20% glycerol and 200mM NaCl.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MEERGDSEPT PGCSGLGPDG VRGFGDGGGA PSWAPEDAWM GTHPKYLEMM ELDIGDATHV YVAFLVYLDL MESKSWHEVN CVGLPELQLI CLVGTEIEGE GLQTVVPTPI TASLSHNRIR EILKASRKLQ GDPDLPMSFT LAIVESDSTI VYYKLTDGFM LPDPQNISLR R

tRNA-splicing endonuclease subunit Sen15 (TSEN15) is a member of the SEN15 family. tRNA splicing is an essential process required for cell growth and division. TSEN15 is a subunit of the tRNA splicing endonuclease. TSEN15 cleaves pre-tRNA at the 5' and 3' splice sites to release the intron.

TSEN15 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 191 amino acids (1-171 a.a.) and having a molecular mass of 20.9kDa. The TSEN15 is purified by proprietary chromatographic techniques.

UniProt Protein Function:Non-catalytic subunit of the tRNA-splicing endonuclease complex, a complex responsible for identification and cleavage of the splice sites in pre-tRNA. It cleaves pre-tRNA at the 5' and 3' splice sites to release the intron. The products are an intron and two tRNA half-molecules bearing 2',3' cyclic phosphate and 5'-OH termini (PubMed:15109492, PubMed:27392077). There are no conserved sequences at the splice sites, but the intron is invariably located at the same site in the gene, placing the splice sites an invariant distance from the constant structural features of the tRNA body. The tRNA splicing endonuclease is also involved in mRNA processing via its association with pre-mRNA 3'-end processing factors, establishing a link between pre-tRNA splicing and pre-mRNA 3'-end formation, suggesting that the endonuclease subunits function in multiple RNA-processing events (PubMed:15109492).
UniProt Protein Details:
NCBI Summary:This gene encodes a subunit of the tRNA splicing endonuclease, which catalyzes the removal of introns from tRNA precursors. Alternative splicing results in multiple transcript variants. There is a pseudogene of this gene on chromosome 17. [provided by RefSeq, Jul 2014]
UniProt Code:Q8WW01
NCBI GenInfo Identifier:24308390
NCBI Gene ID:116461
NCBI Accession:NP_443197
UniProt Secondary Accession:Q8WW01,Q9BZQ5, B4DKP0
UniProt Related Accession:Q8WW01
Molecular Weight:20.9kDa (191aa), confirmed by MALDI-TOF
NCBI Full Name:tRNA-splicing endonuclease subunit Sen15 isoform 1
NCBI Synonym Full Names:tRNA splicing endonuclease subunit 15
NCBI Official Symbol:TSEN15��
NCBI Official Synonym Symbols:PCH2F; sen15; C1orf19��
NCBI Protein Information:tRNA-splicing endonuclease subunit Sen15
UniProt Protein Name:tRNA-splicing endonuclease subunit Sen15
UniProt Synonym Protein Names:SEN15 homolog; HsSEN15; tRNA-intron endonuclease Sen15
Protein Family:tRNA-splicing endonuclease
UniProt Gene Name:TSEN15��
UniProt Entry Name:
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