Cytokines Recombinant Proteins

Human TPO Recombinant Protein (RPPB1050)

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RPPB1050

Description

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Product Name:Human TPO Recombinant Protein
Product Code:RPPB1050
Size:5µg
Species:Human
Target:TPO
Synonyms:MPL Ligand, THCYT1, MPLLG, TPO, ML, ThrombopoietinNirs Variant 1, MKCSF, Thrombopoietin, Myeloproliferative Leukemia VirusOncogene Ligand, Megakaryocyte Growth and Development Factor, MegakaryocyteColony-Stimulating Factor, C-Mpl Ligand, MGDF, Megakaryocyte StimulatingFactor, Prepro-Thrombopoietin.
Source:Chinese Hamster Ovary Cells (CHO)
Physical Appearance:Sterile Filtered clear solution.
Formulation:TPO protein solution contains phosphate buffered saline (pH7.4) and 2% albumin.
Stability:Thrombopoietin although stable at room temperature for 1 week, should be stored between 2-8°C.
Purity:Greater than 98% as determined by SDS-PAGE.
Biological Activity:The ED50 as determined by the dose-dependent stimulation of MO7e cells corresponding to a specific activity of 3x105 units/mg.

Thrombopoietin is a glycoprotein hormone produced mainly by the liver and the kidney which regulates the production of platelets by the bone marrow. TPO stimulates the production as well as differentiation of megakaryocytes, the bone marrow cells which fragment into large numbers of platelets.

Thrombopoietin Human Recombinant produced in CHO cells has a molecular weight range of 80-90kDa due to glycosylation. The TPO is purified by proprietary chromatographic techniques.

UniProt Protein Function:THPO: Lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from their committed progenitor cells. It acts at a late stage of megakaryocyte development. It may be the major physiological regulator of circulating platelets. Defects in THPO are the cause of thrombocythemia type 1 (THCYT1). A myeloproliferative disorder characterized by elevated platelet levels due to sustained proliferation of megakaryocytes, and frequently lead to thrombotic and haemorrhagic complications. Belongs to the EPO/TPO family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Cell cycle regulation; Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function:growth factor activity; hormone activity; cytokine activity

Biological Process: positive regulation of protein kinase B signaling cascade; cell proliferation; platelet activation; multicellular organismal development; myeloid cell differentiation; positive regulation of megakaryocyte differentiation; positive regulation of protein amino acid phosphorylation; blood coagulation

Disease: Thrombocythemia 1

NCBI Summary:Megakaryocytopoiesis is the cellular development process that leads to platelet production. The main functional protein encoded by this gene is a humoral growth factor that is necessary for megakaryocyte proliferation and maturation, as well as for thrombopoiesis. This protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Mutations in this gene are the cause of thrombocythemia 1. Alternative promoter usage and differential splicing result in multiple transcript variants differing in the 5' UTR and/or coding region. Multiple AUG codons upstream of the main open reading frame (ORF) have been identified, and these upstream AUGs inhibit translation of the main ORF at different extent. [provided by RefSeq, Feb 2014]
UniProt Code:P40225
NCBI GenInfo Identifier:730982
NCBI Gene ID:7066
NCBI Accession:P40225.1
UniProt Related Accession:P40225
Molecular Weight:
NCBI Full Name:Thrombopoietin
NCBI Synonym Full Names:thrombopoietin
NCBI Official Symbol:THPO��
NCBI Official Synonym Symbols:ML; TPO; MGDF; MKCSF; MPLLG; THCYT1��
NCBI Protein Information:thrombopoietin
UniProt Protein Name:Thrombopoietin
UniProt Synonym Protein Names:C-mpl ligand; ML; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; MGDF; Myeloproliferative leukemia virus oncogene ligand
Protein Family:Thyroid peroxidase
UniProt Gene Name:THPO��
UniProt Entry Name:TPO_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P40225
Research Area:
Cytokines
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