Human TPM3 Recombinant Protein (RPPB4991)
- SKU:
- RPPB4991
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P06753
Description
| Product Name: | Human TPM3 Recombinant Protein |
| Product Code: | RPPB4991 |
| Size: | 10µg |
| Species: | Human |
| Target: | TPM3 |
| Synonyms: | Tropomyosin alpha-3 chain, Gamma-tropomyosin, Tropomyosin-3, Tropomyosin-5, hTM5, TPM3, TM3, TM5, TRK, CFTD, NEM1, TM-5, TM30, TM30nm, TPMsk3, hscp30, OK/SW-cl.5. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | TPM3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol, 0.1M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMAGITT IEAVKRKIQV LQQQADDAEE RAERLQREVE GERRAREQAE AEVASLNRRI QLVEEELDRA QERLATALQK LEEAEKAADE SERGMKVIEN RALKDEEKME LQEIQLKEAK HIAEEADRKY EEVARKLVII EGDLERTEER AELAESRCRE� MDEQIRLMDQ NLKCLSAAEE KYSQKEDKYE EEIKILTDKL KEAETRAEFA ERSVAKLEKT IDDLEDKLKC TKEEHLCTQR MLDQTLLDLN EM |
Tropomyosin alpha-3 chain (TPM3) belongs to the tropomyosin family of actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosins are dimers of coiled-coil proteins which polymerize end-to-end along the major groove in most actin filaments. Tropomyosins give stability to the filaments and regulate access of other actin-binding proteins. In muscle cells, tropomyosins regulate muscle contraction by controlling the binding of myosin heads to the actin filament. Mutations in the TPM3 gene cause autosomal dominant nemaline myopathy, and oncogenes formed by chromosomal translocations involving this locus are linked with cancer.
TPM3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 272 amino acids (1-248 a.a.) and having a molecular mass of 31.6kDa. TPM3 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TPM3: a cytoskeletal protein that binds to actin filaments in muscle and nonmuscle cells. Plays a central role, in association with the troponin complex, in the calcium dependent regulation of vertebrate striated muscle contraction. Smooth muscle contraction is regulated by interaction with caldesmon. In non-muscle cells is implicated in stabilizing cytoskeleton actin filaments. Defects in TPM3 are a cause of nemaline myopathy type 1 (NEM1). Three alternatively spliced isoforms have been described. |
| UniProt Protein Details: | Protein type:Contractile; Cytoskeletal; Oncoprotein; Actin-binding; Motor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 1q21.2 Cellular Component: cortical cytoskeleton; filamentous actin; cytoskeleton; growth cone; stress fiber; podosome; cytosol; muscle thin filament tropomyosin; cleavage furrow Molecular Function:actin binding Biological Process: muscle contraction; cell motility; muscle filament sliding Disease: Nemaline Myopathy 1; Myopathy, Congenital, With Fiber-type Disproportion |
| NCBI Summary: | This gene encodes a member of the tropomyosin family of actin-binding proteins. Tropomyosins are dimers of coiled-coil proteins that provide stability to actin filaments and regulate access of other actin-binding proteins. Mutations in this gene result in autosomal dominant nemaline myopathy and other muscle disorders. This locus is involved in translocations with other loci, including anaplastic lymphoma receptor tyrosine kinase (ALK) and neurotrophic tyrosine kinase receptor type 1 (NTRK1), which result in the formation of fusion proteins that act as oncogenes. There are numerous pseudogenes for this gene on different chromosomes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013] |
| UniProt Code: | P06753 |
| NCBI GenInfo Identifier: | 519668659 |
| NCBI Gene ID: | 7170 |
| NCBI Accession: | P06753.2 |
| UniProt Secondary Accession: | P06753,P12324, Q2QD06, Q5VU58, Q5VU63, Q5VU66, Q5VU71 Q5VU72, Q8TCG3, Q969Q2, Q9NQH8, D3DV71, |
| UniProt Related Accession: | P06753 |
| Molecular Weight: | |
| NCBI Full Name: | Tropomyosin alpha-3 chain |
| NCBI Synonym Full Names: | tropomyosin 3 |
| NCBI Official Symbol: | TPM3�� |
| NCBI Official Synonym Symbols: | TM3; TM5; TRK; CFTD; NEM1; TM-5; TM30; CAPM1; TM30nm; TPMsk3; hscp30; HEL-189; HEL-S-82p; OK/SW-cl.5�� |
| NCBI Protein Information: | tropomyosin alpha-3 chain; tropomyosin-5; tropomyosin gamma; cytoskeletal tropomyosin TM30; epididymis luminal protein 189; alpha-tropomyosin, slow skeletal; heat-stable cytoskeletal protein 30 kDa; epididymis secretory sperm binding protein Li 82p |
| UniProt Protein Name: | Tropomyosin alpha-3 chain |
| UniProt Synonym Protein Names: | Gamma-tropomyosin; Tropomyosin-3; Tropomyosin-5; hTM5 |
| Protein Family: | Tropomyosin |
| UniProt Gene Name: | TPM3�� |
| UniProt Entry Name: | TPM3_HUMAN |
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