Enzymes Recombinant Proteins
Human TPI1 Recombinant Protein (RPPB2339)
- SKU:
- RPPB2339
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P60174
- Research Area:
- Enzymes
Description
| Product Name: | Human TPI1 Recombinant Protein |
| Product Code: | RPPB2339 |
| Size: | 10µg |
| Species: | Human |
| Target: | TPI1 |
| Synonyms: | TPI, TIM, Triosephosphate Isomerase 1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | TPI1 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 1mM DTT and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 95% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MAPSRKFFVG GNWKMNGRKQ SLGELIGTLN AAKVPADTEV VCAPPTAYID FARQKLDPKI AVAAQNCYKV TNGAFTGEIS PGMIKDCGAT WVVLGHSERR HVFGESDELI GQKVAHALAE GLGVIACIGE KLDEREAGIT EKVVFEQTKV IADNVKDWSK VVLAYEPVWA IGTGKTATPQ QAQEVHEKLR GWLKSNVSDA VAQSTRIIYG GSVTGATCKE LASQPDVDGF LVGGASLKPE FVDIINAKQ |
| Biological Activity: | Specific activity is > 3000 units/mg, in which oneunit will convert 1.0 umole of D-glyceraldehyde-3-phosphate to dihydroxyacetonephosphate per minute at pH 7.5 at 25C. |
TPI1 is one of the triosephosphate isomerase family. TPI1 catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in TPI1 causes triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder which is the most severe clinical disorder of glycolysis and is related to neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection.
TPI1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 269 amino acids (1-249a.a.) and having a molecular mass of 28.8kDa.TPI1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TPI1: Defects in TPI1 are the cause of triosephosphate isomerase deficiency (TPI deficiency). TPI deficiency is an autosomal recessive disorder. It is the most severe clinical disorder of glycolysis. It is associated with neonatal jaundice, chronic hemolytic anemia, progressive neuromuscular dysfunction, cardiomyopathy and increased susceptibility to infection. Belongs to the triosephosphate isomerase family. 2 isoforms of the human protein are produced by alternative promoter. |
| UniProt Protein Details: | Protein type:EC 5.3.1.1; Carbohydrate Metabolism - glycolysis and gluconeogenesis; Isomerase; Carbohydrate Metabolism - inositol phosphate; Carbohydrate Metabolism - fructose and mannose Chromosomal Location of Human Ortholog: 12p13 Cellular Component: extracellular space; cytosol; nucleus Molecular Function:protein binding; triose-phosphate isomerase activity Biological Process: pentose-phosphate shunt; glycolysis; multicellular organismal development; carbohydrate metabolic process; glucose metabolic process; pathogenesis; gluconeogenesis Disease: Triosephosphate Isomerase Deficiency |
| NCBI Summary: | This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate (G3P) and dihydroxy-acetone phosphate (DHAP) in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009] |
| UniProt Code: | P60174 |
| NCBI GenInfo Identifier: | 353526311 |
| NCBI Gene ID: | 7167 |
| NCBI Accession: | P60174.3 |
| UniProt Secondary Accession: | P60174,P00938, Q6FHP9, Q6IS07, Q8WWD0, Q96AG5, B7Z5D8 D3DUS9, |
| UniProt Related Accession: | P60174 |
| Molecular Weight: | 249 |
| NCBI Full Name: | Triosephosphate isomerase |
| NCBI Synonym Full Names: | triosephosphate isomerase 1 |
| NCBI Official Symbol: | TPI1�� |
| NCBI Official Synonym Symbols: | TIM; TPI; TPID; HEL-S-49�� |
| NCBI Protein Information: | triosephosphate isomerase; triose-phosphate isomerase; epididymis secretory protein Li 49 |
| UniProt Protein Name: | Triosephosphate isomerase |
| UniProt Synonym Protein Names: | Triose-phosphate isomerase |
| UniProt Gene Name: | TPI1�� |
| UniProt Entry Name: | TPIS_HUMAN |
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