Cytokines Recombinant Proteins

Human TNF b Recombinant Protein (RPPB1004)

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RPPB1004

Description

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Product Name:Human TNF b Recombinant Protein
Product Code:RPPB1004
Size:20µg
Species:Human
Target:TNF b
Synonyms:Lymphotoxin-alpha, LT-alpha, TNF-beta, Tumor necrosis factor ligand superfamily member 1, LTA, LT, TNFB, TNFSF1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:TNF-b protein solution (0.25mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MLPGVGLTPS AAQTARQHPK MHLAHSTLKP AAHLIGDPSK QNSLLWRANT DRAFLQDGFS LSNNSLLVPT SGIYFVYSQV VFSGKAYSPK ATSSPLYLAH EVQLFSSQYP FHVPLLSSQK MVYPGLQEPW LHSMYHGAAF QLTQGDQLST HTDGIPHLVL SPSTVFFGAF AL
Biological Activity:Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is <0.3 ng/ml.

TNF-b is a potent multifunctional cytokine produced mainly by activated T and B lymphocytes. The protein has been shown to have a specific potent cytotoxic activity on tumor cells and a variety of other target cells as well as being a mediator of inflammation and immune function.

TNF-b Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 192 amino acids (35-205 a.a.) and having a molecular mass of 20.9kDa. TNF-b is fused to a 21 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:LTA: Cytokine that in its homotrimeric form binds to TNFRSF1A/TNFR1, TNFRSF1B/TNFBR and TNFRSF14/HVEM. In its heterotrimeric form with LTB binds to TNFRSF3/LTBR. Lymphotoxin is produced by lymphocytes and cytotoxic for a wide range of tumor cells in vitro and in vivo. Genetic variations in LTA are a cause of susceptibility psoriatic arthritis (PSORAS). PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). Belongs to the tumor necrosis factor family.
UniProt Protein Details:

Protein type:Cytokine; Apoptosis; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6p21.3

Cellular Component: extracellular space; membrane

Molecular Function:cytokine activity; tumor necrosis factor receptor binding; receptor binding

Biological Process: response to drug; positive regulation of humoral immune response mediated by circulating immunoglobulin; apoptosis; positive regulation of apoptosis; response to lipopolysaccharide; signal transduction; lymph node development; humoral immune response; defense response to Gram-positive bacterium; positive regulation of interferon-gamma production; cell-cell signaling; negative regulation of fibroblast proliferation; response to hypoxia; positive regulation of chronic inflammatory response to antigenic stimulus; response to nutrient

Disease: Psoriatic Arthritis, Susceptibility To; Myocardial Infarction, Susceptibility To; Leprosy, Susceptibility To, 4

NCBI Summary:The encoded protein, a member of the tumor necrosis factor family, is a cytokine produced by lymphocytes. The protein is highly inducible, secreted, and forms heterotrimers with lymphotoxin-beta which anchor lymphotoxin-alpha to the cell surface. This protein also mediates a large variety of inflammatory, immunostimulatory, and antiviral responses, is involved in the formation of secondary lymphoid organs during development and plays a role in apoptosis. Genetic variations in this gene are associated with susceptibility to leprosy type 4, myocardial infarction, non-Hodgkin's lymphoma, and psoriatic arthritis. Alternatively spliced transcript variants have been observed for this gene. [provided by RefSeq, Jul 2012]
UniProt Code:P01374
NCBI GenInfo Identifier:135940
NCBI Gene ID:4049
NCBI Accession:P01374.2
UniProt Secondary Accession:P01374,Q8N4C3, Q9UKS8,
UniProt Related Accession:P01374
Molecular Weight:22,297 Da
NCBI Full Name:Lymphotoxin-alpha
NCBI Synonym Full Names:lymphotoxin alpha
NCBI Official Symbol:LTA��
NCBI Official Synonym Symbols:LT; TNFB; TNFSF1��
NCBI Protein Information:lymphotoxin-alpha; LT-alpha; TNF-beta; TNF superfamily, member 1; tumor necrosis factor beta; tumor necrosis factor ligand superfamily member 1
UniProt Protein Name:Lymphotoxin-alpha
UniProt Synonym Protein Names:TNF-beta; Tumor necrosis factor ligand superfamily member 1
Protein Family:Lymphotoxin
UniProt Gene Name:LTA��
UniProt Entry Name:TNFB_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P01374
Research Area:
Cytokines
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