Growth Factors & Cytokines Recombinant Proteins
Human TNF-alpha/TNFA Recombinant Protein (RPES1643)
- SKU:
- RPES1643
Description
Human TNF-alpha/TNFA Recombinant Protein
Tumor Necrosis Factor-? (TNF-?) is secreted by macrophages; monocytes; neutrophils; T-cells; and NK-cells following stimulation by bacterial LPS. Cells expressing CD4 secrete TNF-? while cells that express CD8 secrete little or no TNF-?. Synthesis of TNF-? can be induced by many different stimuli including interferons; IL2; and GM-CSF. The clinical use of the potent anti-tumor activity of TNF-? has been limited by the proinflammatory side effects such as fever; dose-limiting hypotension; hepatotoxicity; intravascular thrombosis; and hemorrhage. Designing clinically applicable TNF-? mutants with low systemic toxicity has been of intense pharmacological interest. Human TNF-? that binds to murine TNF-R55 but not murine TNF-R7; exhibits retained anti-tumor activity and reduced systemic toxicity in mice compared with murine TNF-?; which binds to both murine TNF receptors. Based on these results; many TNF-? mutants that selectively bind to TNF-R55 have been designed. These mutants displayed cytotoxic activities on tumor cell lines in vitro and have exhibited lower systemic toxicity in vivo. Recombinant Human TNF-? High Active Mutant differs from the wild-type by amino acid subsitution of amino acids 1-7 with Arg8; Lys9; Arg10 and Phe157. This mutant form has been shown to have increased activity with less inflammatory side effects in vivo.
| Product Name: | Human TNF-alpha/TNFA Recombinant Protein (RPES1643) |
| Product Code: | RPES1643 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2 |
| Accession: | P01375 |
| Sequence: | Val77-Leu233 |
| Fusion tag: | C-6His |
| Activity: | Measured in a cytotoxicity assay using L?929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is 10-40 pg/ml. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed with a 6His tag at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 18.5 kDa |
| AP Mol Mass: | 16 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;pH7.4. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
Additional Information
Product Type: |
Recombinant Protein |
Species: |
Human |
Research Area: |
Growth Factors & Cytokines |
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Growth Factors & Cytokines Recombinant Proteins