Human TIRAP Recombinant Protein (RPPB4966)
- SKU:
- RPPB4966
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P58753
Description
| Product Name: | Human TIRAP Recombinant Protein |
| Product Code: | RPPB4966 |
| Size: | 10µg |
| Species: | Human |
| Target: | TIRAP |
| Synonyms: | Toll-interleukin 1 receptor (TIR) domain containing adaptor protein, adapter protein wyatt, BACTS1, Mal, wyatt, Toll-like receptor adaptor protein, TIR domain-containing adapter protein, MyD88 adapter-like protein. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The TIRAP solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0), 200mM NaCl, 2mM DTT and 20% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMASSTSL PAPGSRPKKP LGKMADWFRQ TLLKKPKKRP NSPESTSSDA SQPTSQDSPL PPSLSSVTSP SLPPTHASDS GSSRWSKDYD VCVCHSEEDL VAAQDLVSYL EGSTASLRCF LQLRDATPGG AIVSELCQAL SSSHCRVLLI TPGFLQDPWC KYQMLQALTE APGAEGCTIP LLSGLSRAAY PPELRFMYYV DGRGPDGGFR QVKEAVMRYL QTLS |
Toll-interleukin1 receptor (TIR) domain containing adaptor protein (TIRAP) contains 1 TIR domain. TIRAP is an adapter molecule linked with toll-like receptors. TIRAP is required in TLR2 and TLR4 signaling pathways in the innate immune response. TIRAP activates NF-kappa-B, MAPK1, MAPK3 and JNK, which subsequently results in cytokine secretion and the inflammatory response. TIRAP is highly expressed in the liver, kidney, spleen, skeletal muscle and heart. TIRAP is also found in peripheral blood leukocytes, lung, placenta, small intestine, thymus, colon and brain.
TIRAP Human Recombinant produced in E. coli is a single polypeptide chain containing 244 amino acids (1-221) and having a molecular mass of 26.3 kDa.TIRAP is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TIRAP: an adapter protein involved in the TLR4 signaling pathway in the innate immune response. Acts via IRAK2 and TRAF-6, leading to the activation of NF-kappa-B, ERK1, ERK2 and JNK, resulting in cytokine secretion and the inflammatory response. Homodimerizes. Also forms heterodimers with MyD88. Binds to TLR4 and IRAK2 via their respective TIR domains. Binds to PKR and TBK1. Does not interact with IRAK1, nor TLR9. Genetic variations in TIRAP can influence susceptibility or resistance to infectious diseases, including invasive pneumococcal disease, malaria and tuberculosis. Three isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 11q24.2 Cellular Component: nucleoplasm; intercellular bridge; endocytic vesicle; cytoplasm; plasma membrane; cytosol Molecular Function:protein binding, bridging; protein binding; phosphatidylinositol-4,5-bisphosphate binding; protein homodimerization activity; protein kinase C binding; protein heterodimerization activity Biological Process: I-kappaB kinase/NF-kappaB cascade; positive regulation of interleukin-12 production; positive regulation of interleukin-6 biosynthetic process; positive regulation of JNK cascade; response to lipopolysaccharide; activation of NF-kappaB transcription factor; positive regulation of interleukin-8 production; cell surface receptor linked signal transduction; positive regulation of interleukin-15 production; positive regulation of B cell proliferation; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; positive regulation of toll-like receptor 3 signaling pathway; positive regulation of toll-like receptor 4 signaling pathway; positive regulation of tumor necrosis factor production; positive regulation of toll-like receptor 2 signaling pathway; regulation of innate immune response; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; defense response to Gram-positive bacterium; toll-like receptor signaling pathway; innate immune response; myeloid cell differentiation; regulation of interferon-beta production Disease: Invasive Pneumococcal Disease, Recurrent Isolated, 1; Bacteremia, Susceptibility To, 1; Mycobacterium Tuberculosis, Susceptibility To; Malaria, Susceptibility To |
| NCBI Summary: | The innate immune system recognizes microbial pathogens through Toll-like receptors (TLRs), which identify pathogen-associated molecular patterns. Different TLRs recognize different pathogen-associated molecular patterns and all TLRs have a Toll-interleukin 1 receptor (TIR) domain, which is responsible for signal transduction. The protein encoded by this gene is a TIR adaptor protein involved in the TLR4 signaling pathway of the immune system. It activates NF-kappa-B, MAPK1, MAPK3 and JNK, which then results in cytokine secretion and the inflammatory response. Alternative splicing of this gene results in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P58753 |
| NCBI GenInfo Identifier: | 50403750 |
| NCBI Gene ID: | 114609 |
| NCBI Accession: | P58753.2 |
| UniProt Secondary Accession: | P58753,Q56UH9, Q56UI0, Q8N5E5, B3KW65, |
| UniProt Related Accession: | P58753 |
| Molecular Weight: | 221 |
| NCBI Full Name: | Toll/interleukin-1 receptor domain-containing adapter protein |
| NCBI Synonym Full Names: | toll-interleukin 1 receptor (TIR) domain containing adaptor protein |
| NCBI Official Symbol: | TIRAP�� |
| NCBI Official Synonym Symbols: | Mal; wyatt; BACTS1; MyD88-2�� |
| NCBI Protein Information: | toll/interleukin-1 receptor domain-containing adapter protein; adapter protein wyatt; adaptor protein Wyatt; MyD88 adapter-like protein; Toll-like receptor adaptor protein |
| UniProt Protein Name: | Toll/interleukin-1 receptor domain-containing adapter protein |
| UniProt Synonym Protein Names: | Adaptor protein Wyatt; MyD88 adapter-like protein; MyD88-2 |
| Protein Family: | Toll/interleukin-1 receptor domain-containing adapter protein |
| UniProt Gene Name: | TIRAP�� |
| UniProt Entry Name: | TIRAP_HUMAN |
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