Enzymes Recombinant Proteins
Human TIMP2 Recombinant Protein (RPPB2326)
- SKU:
- RPPB2326
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P16035
- Research Area:
- Enzymes
Description
| Product Name: | Human TIMP2 Recombinant Protein |
| Product Code: | RPPB2326 |
| Size: | 10µg |
| Species: | Human |
| Target: | TIMP2 |
| Synonyms: | TIMP metallopeptidase inhibitor 2, CSC-21K, tissue inhibitor of metalloproteinase 2, TIMP-2, metalloproteinase inhibitor 2. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The TIMP2 protein was lyophilized after extensive dialysis against PBS. |
| Solubility: | It is recommended to reconstitute the lyophilized TIMP2 in sterile assay buffer (50mM Tris, 10mM CaCl2, 150mM NaCl, 0.05% Brij-35, PH 7.5) not less than 100�g/ml. |
| Stability: | Lyophilized TIMP2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Biological Activity: | The IC50 of 3 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2. |
TIMP2 belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases that take part in degradation of the extracellular matrix. Besides having an inhibitory role against metalloproteinases, the encoded protein has a exclusive part among TIMP family members in its capability to directly suppress the proliferation of endothelial cells. Consequently, the encoded protein is crucial to the conservation of tissue homeostasis by suppressing the production of quiescent tissues as an answer to angiogenic factors, and by inhibiting protease activity in tissues undergoing renovation of the extracellular matrix.
TIMP2 Human Recombinant produced in HEK-293 cells is a secreted protein with the sequence of Human TIMP-2 (amino acids Cys27-Pro220) and is fused to a polyhistidine tag at the C-terminus.
| UniProt Protein Function: | TIMP2: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-13, MMP-14, MMP-15, MMP-16 and MMP-19. Interacts (via the C-terminal) with MMP2 (via the C- terminal PEX domain); the interaction inhibits the MMP2 activity. Down-regulated by TGFB1. Belongs to the protease inhibitor I35 (TIMP) family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide; Extracellular matrix; Motility/polarity/chemotaxis; Cell development/differentiation; Inhibitor Chromosomal Location of Human Ortholog: 17q25 Cellular Component: extracellular space; proteinaceous extracellular matrix; growth cone; cell surface; cell soma; extracellular region; basement membrane Molecular Function:integrin binding; metalloendopeptidase inhibitor activity; protein binding; protease binding; metal ion binding; enzyme activator activity Biological Process: response to drug; regulation of Rap protein signal transduction; negative regulation of metalloenzyme activity; extracellular matrix organization and biogenesis; central nervous system development; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; negative regulation of cell proliferation; positive regulation of MAPKKK cascade; response to cytokine stimulus; negative regulation of Ras protein signal transduction; negative regulation of mitotic cell cycle; positive regulation of adenylate cyclase activity; positive regulation of neuron differentiation; aging |
| NCBI Summary: | This gene is a member of the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases, a group of peptidases involved in degradation of the extracellular matrix. In addition to an inhibitory role against metalloproteinases, the encoded protein has a unique role among TIMP family members in its ability to directly suppress the proliferation of endothelial cells. As a result, the encoded protein may be critical to the maintenance of tissue homeostasis by suppressing the proliferation of quiescent tissues in response to angiogenic factors, and by inhibiting protease activity in tissues undergoing remodelling of the extracellular matrix. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P16035 |
| NCBI GenInfo Identifier: | 135854 |
| NCBI Gene ID: | 7077 |
| NCBI Accession: | P16035.2 |
| UniProt Secondary Accession: | P16035,Q16121, Q93006, Q9UDF7, |
| UniProt Related Accession: | P16035,Q96MC4 |
| Molecular Weight: | 24,399 Da |
| NCBI Full Name: | Metalloproteinase inhibitor 2 |
| NCBI Synonym Full Names: | TIMP metallopeptidase inhibitor 2 |
| NCBI Official Symbol: | TIMP2�� |
| NCBI Official Synonym Symbols: | DDC8; CSC-21K�� |
| NCBI Protein Information: | metalloproteinase inhibitor 2; TIMP-2; tissue inhibitor of metalloproteinase 2; tissue inhibitor of metalloproteinases 2 |
| UniProt Protein Name: | Metalloproteinase inhibitor 2 |
| UniProt Synonym Protein Names: | CSC-21K; Tissue inhibitor of metalloproteinases 2; TIMP-2 |
| Protein Family: | Metalloproteinase inhibitor |
| UniProt Gene Name: | TIMP2�� |
| UniProt Entry Name: | TIMP2_HUMAN |
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