Enzymes Recombinant Proteins
Human TIMP1 Recombinant Protein (RPPB2320)
- SKU:
- RPPB2320
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P01033
- Research Area:
- Enzymes
Description
| Product Name: | Human TIMP1 Recombinant Protein |
| Product Code: | RPPB2320 |
| Size: | 10µg |
| Species: | Human |
| Target: | TIMP1 |
| Synonyms: | Metalloproteinase inhibitor 1, Tissue inhibitor of metalloproteinases, TIMP-1, Erythroid-potentiating activity, EPA, TIMP1, CLGI, TIMP, EPO, HCI, FLJ90373. |
| Source: | HEK293 Cells |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | The TIMP1 protein was lyophilized after extensive dialysis against PBS. |
| Solubility: | It is recommended to reconstitute the lyophilized TIMP1 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized TIMP1 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TIMP1 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Purity: | Greater than 95.0% as determined by SDS-PAGE. |
| Biological Activity: | The IC50 of 2.5-4 nM is measured by its ability to inhibit recombinant human MMP-2 cleavage of the colorimetric peptide substrate, Mca-PLGL-DpaAR-NH2. |
TIMP1 is a member of the TIMP family. TIMP1 is an inducible glycoprotein produced by various cell types. The TIMP1 glycoprotein is a natural inhibitor of the matrix metalloproteinases, which a group of peptidases involved in degradation of the extracellular matrix. TIMP1 binds in a reversible mode to MMPs, with regions in the N-terminal domain binding to the MMP substrate-binding site. On top of its inhibitory function against most of the known MMPs, TIMP1 is able to promote cell proliferation in a broad range of cell types, and may also have an anti-apoptotic role. Furthermore, TIMP1 has erthyroid-potentiating activity via translocation to the nucleus and also inhibits apoptosis in B-cells. The TIMP1 gene is situated within intron 6 of the synapsin I gene and is transcribed in the opposite direction. TIMP1 activity is dependent on the existence of disulfide bonds. TIMP1 transcription is extremely inducible in reaction to many cytokines and hormones. Increased TIMP1 levels are connecte
TIMP1 Human Recombinant produced in HEK-293 cells is a secreted protein with the sequence of Human TIMP-1 (amino acids Cys24-Ala207) and fused to a polyhistidine tag at the C-terminus.
| UniProt Protein Function: | TIMP1: Complexes with metalloproteinases (such as collagenases) and irreversibly inactivates them by binding to their catalytic zinc cofactor. Also mediates erythropoiesis in vitro; but, unlike IL-3, it is species-specific, stimulating the growth and differentiation of only human and murine erythroid progenitors. Known to act on MMP-1, MMP-2, MMP-3, MMP-7, MMP-8, MMP-9, MMP-10, MMP-11, MMP-12, MMP-13 and MMP-16. Does not act on MMP-14. Belongs to the protease inhibitor I35 (TIMP) family. |
| UniProt Protein Details: | Protein type:Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: Xp11.3-p11.23 Cellular Component: extracellular space; proteinaceous extracellular matrix; extracellular region; basement membrane Molecular Function:metalloendopeptidase inhibitor activity; protein binding; growth factor activity; protease binding; metal ion binding; cytokine activity Biological Process: response to peptide hormone stimulus; platelet activation; extracellular matrix organization and biogenesis; negative regulation of metalloenzyme activity; response to hormone stimulus; negative regulation of membrane protein ectodomain proteolysis; extracellular matrix disassembly; platelet degranulation; response to cytokine stimulus; positive regulation of cell proliferation; blood coagulation; aging; negative regulation of apoptosis |
| NCBI Summary: | This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P01033 |
| NCBI GenInfo Identifier: | 135850 |
| NCBI Gene ID: | 7076 |
| NCBI Accession: | P01033.1 |
| UniProt Secondary Accession: | P01033,Q14252, Q9UCU1, |
| UniProt Related Accession: | P01033 |
| Molecular Weight: | 23,171 Da |
| NCBI Full Name: | Metalloproteinase inhibitor 1 |
| NCBI Synonym Full Names: | TIMP metallopeptidase inhibitor 1 |
| NCBI Official Symbol: | TIMP1�� |
| NCBI Official Synonym Symbols: | EPA; EPO; HCI; CLGI; TIMP�� |
| NCBI Protein Information: | metalloproteinase inhibitor 1; TIMP-1; collagenase inhibitor; erythroid potentiating activity; erythroid-potentiating activity; fibroblast collagenase inhibitor; tissue inhibitor of metalloproteinases 1 |
| UniProt Protein Name: | Metalloproteinase inhibitor 1 |
| UniProt Synonym Protein Names: | Erythroid-potentiating activity; EPA; Fibroblast collagenase inhibitor; Collagenase inhibitor; Tissue inhibitor of metalloproteinases 1; TIMP-1 |
| Protein Family: | Metalloproteinase inhibitor |
| UniProt Gene Name: | TIMP1�� |
| UniProt Entry Name: | TIMP1_HUMAN |
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