Signal Transduction Recombinant Proteins
Human Thyroid peroxidase/TPO Recombinant Protein (RPES0835)
- SKU:
- RPES0835
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Signal Transduction
Description
Human Thyroid peroxidase/TPO Recombinant Protein
Thyroid peroxidase is a membrane-bound glycoprotein which belongs to the peroxidase family, XPO subfamily. It contains 1 EGF-like domain and 1 Sushi (CCP/SCR) domain. Thyroid Peroxidase represents one of the main autoantigenic targets in autoimmune thyroid disease of humans. It used to be taken as the formerly so-called `microsomal antigen` several years ago. As an integral membrane glycoprotein it is restricted to the apical plasma membrane of the follicular epithelial cells and comprises two identical subunits of approx 100 kDa molecular weight. Thyroid peroxidase is an enzyme expressed abundantly in the thyroid that liberates iodine for addition onto tyrosine residues on thyroglobulin for the production of thyroxine or triiodothyronine, thyroid hormones. Thyroid peroxidase plays a key role in the thyroid hormone biosynthesis by catalysing both the iodination of tyrosyl residues and the coupling of iodotyrosyl residues in thyroglobulin to form precursors of the thyroid hormones T4 and T3.
| Product Name: | Human Thyroid peroxidase/TPO Recombinant Protein (RPES0835) |
| Product Code: | RPES0835 |
| Size: | 20µg |
| Species: | Human |
| Expressed Host: | Baculovirus-Insect Cells |
| Synonyms: | MSA,TDH2A,TPX |
| Accession: | P07202-1 |
| Sequence: | Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr |
| Fusion tag: | C-His |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | A DNA sequence encoding the human TPO (P07202-1) extracellular domain (Met 1-Arg846, 257 Ser/Ala, 725 Pro/Thr) was fused with a polyhistidine tag at the C-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 93.8 kDa |
| AP Mol Mass: | 90-100 kDa |
| Formulation: | Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 7.4, 10% gly |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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