Human Thrombospondin-5/COMP PharmaGenie ELISA Kit (SBRS1207)

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SKU:
SBRS1207
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

system_update_altDatasheet

Human Thrombospondin-5/COMP PharmaGenie ELISA Kit (SBRS1207)

Product SKU:SBRS1207
Size:96T
Application:This ELISA antibody pair recognizes Human Thrombospondin-5.
Uniprot:P49747
Gene ID:1311
Gene Names:COMP
Synonyms:Cartilage oligomeric matrix protein (COMP) (Thrombospondin-5) (TSP5)
Target Species:Human
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.085 ng/ml - 20 ng/ml
Sensitivity:0.085 ng/ml
Recommended Dilution:150 fold
Storage/Stability:May be stored for up to 6 months at 2 to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened Microplate Wells or reagents may be store for up to 1 month at 2 to 8°C. Return unused wells to the pouch containing desiccant pack, reseal along entire edge. Note: the kit can be used within one year if the whole kit is stored at -20°C.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Stop Solution
  4. Assay Diluent(s)
  5. Lyophilized Standard
  6. Biotinylated Detection Antibody
  7. Streptavidin-Conjugated HRP
  8. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human Thrombospondin-5/COMP PharmaGenie ELISA Kit (SBRS1207) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
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