Human Thrombin ELISA Kit (HUFI04750)
- SKU:
- HUFI04750
- Product Type:
- ELISA Kit
- Size:
- 96 Assays
- Uniprot:
- P00734
- Sensitivity:
- 0.938ng/ml
- Range:
- 1.563-100ng/ml
- ELISA Type:
- Sandwich
- Synonyms:
- Thrombin
- Reactivity:
- Human
Description
Human Thrombin ELISA
F2 (Coagulation Factor II, Thrombin) is a coagulation factor that is produced in the liver. It helps to form blood clots by converting fibrinogen into fibrin. Fibrin is an essential component of blood clots, and helps to stop bleeding. F2 is used to treat or prevent bleeding in people with low levels of coagulation factors, or who are undergoing surgery. Diseases associated with F2 include Prothrombin Deficiency, Congenital and Thrombophilia Due To Thrombin Defect. Among F2 related pathways are Folate Metabolism and Formation of Fibrin Clot (Clotting Cascade).
| Product Name: | Human Thrombin ELISA Kit |
| Product Code: | HUFI04750 |
| Size: | 96 Assays |
| Alias: | Thrombin ELISA Kit |
| Detection method: | Sandwich ELISA, Double Antibody |
| Application: | This immunoassay kit allows for the in vitro quantitative determination of Human Thrombin concentrations in serum plasma and other biological fluids. |
| Sensitivity: | < 0.188ng/ml |
| Range: | 0.313-20ng/ml |
| Storage: | 4°C for 6 months |
| Note: | For Research Use Only |
| Recovery: | Matrices listed below were spiked with certain level of Human Thrombin and the recovery rates were calculated by comparing the measured value to the expected amount of Human Thrombin in samples. Enquire for more information. |
| Linearity: | The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human Thrombin and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected. Enquire for more information. |
| CV(%): | Intra-Assay: CV<8% Inter-Assay: CV<10% |
| Component | Quantity | Storage |
| ELISA Microplate (Dismountable) | 8×12 strips | 4°C for 6 months |
| Lyophilized Standard | 2 | 4°C/-20°C |
| Sample/Standard Dilution Buffer | 20ml | 4°C |
| Biotin-labeled Antibody(Concentrated) | 120ul | 4°C (Protect from light) |
| Antibody Dilution Buffer | 10ml | 4°C |
| HRP-Streptavidin Conjugate(SABC) | 120ul | 4°C (Protect from light) |
| SABC Dilution Buffer | 10ml | 4°C |
| TMB Substrate | 10ml | 4°C (Protect from light) |
| Stop Solution | 10ml | 4°C |
| Wash Buffer(25X) | 30ml | 4°C |
| Plate Sealer | 5 | - |
Other materials and equipment required:
- Microplate reader with 450 nm wavelength filter
- Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
- Incubator
- Deionized or distilled water
- Absorbent paper
- Buffer resevoir
| UniProt Protein Function: | Function: Thrombin, which cleaves bonds after Arg and Lys, converts fibrinogen to fibrin and activates factors V, VII, VIII, XIII, and, in complex with thrombomodulin, protein C. Functions in blood homeostasis, inflammation and wound healing. Ref.12 |
| UniProt Protein Details: | Catalytic activity: Selective cleavage of Arg--Gly bonds in fibrinogen to form fibrin and release fibrinopeptides A and B. Subcellular location: Secreted ? § extracellular space. Tissue specificity: Expressed by the liver and secreted in plasma. Fill out our quote form below and a dedicated member of staff will get back to you within one working day! |
