Cell Biology Recombinant Proteins
Human Thioredoxin/TXN Recombinant Protein (RPES0702)
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- SKU:
- RPES0702
- Product Type:
- Recombinant Protein
- Species:
- Human
- Research Area:
- Cell Biology
Description
system_update_altDatasheet
Human Thioredoxin/TXN Recombinant Protein
Thioredoxin (TXN) is a member of the Thioredoxin family. Thioredoxin exists as a disulfide-linked homodimer and contains one Thioredoxin domain. Thioredoxin is up-regulated by ionizing radiation. Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin also plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide.
| Product Name: | Human Thioredoxin/TXN Recombinant Protein (RPES0702) |
| Product Code: | RPES0702 |
| Size: | 10µg |
| Species: | Human |
| Expressed Host: | E.coli |
| Synonyms: | Thioredoxin, Trx, ATL-Derived Factor, ADF, Surface-Associated Sulphydryl Protein, SASP, TXN, TRDX, TRX, TRX1 |
| Accession: | P10599 |
| Sequence: | Met 1-Val105 |
| Fusion tag: | N-6His |
| Activity: | Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation; which can be measured by absorbance at 650 nm. The specific activity is 0.2-1 Abs/min/mg; as measured under the described conditions. |
| Endotoxin: | <1.0 EU per µg as determined by the LAL method. |
| Protein Construction: | Recombinant Human Thioredoxin is produced by our E.coli expression system and the target gene encoding Met1-Val105 is expressed with a 6His tag at the N-terminus. |
| Purity: | > 95 % as determined by reducing SDS-PAGE. |
| Mol Mass: | 13.9 kDa |
| AP Mol Mass: | 14 kDa |
| Formulation: | Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;PH7.2. |
| Shipping: | This product is provided as lyophilized powder which is shipped with ice packs. |
| Stability and Storage: | Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
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