Cell Biology Recombinant Proteins

Human Thioredoxin/TXN Recombinant Protein (RPES0702)

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RPES0702

Description

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Human Thioredoxin/TXN Recombinant Protein

Thioredoxin (TXN) is a member of the Thioredoxin family. Thioredoxin exists as a disulfide-linked homodimer and contains one Thioredoxin domain. Thioredoxin is up-regulated by ionizing radiation. Thioredoxin participates in various redox reactions through the reversible oxidation of its active center dithiol to a disulfide and catalyzes dithiol-disulfide exchange reactions. Thioredoxin also plays a role in the reversible S-nitrosylation of cysteine residues in target proteins; and thereby contributes to the response to intracellular nitric oxide.

Product Name:Human Thioredoxin/TXN Recombinant Protein (RPES0702)
Product Code:RPES0702
Size:10µg
Species:Human
Expressed Host:E.coli
Synonyms:Thioredoxin, Trx, ATL-Derived Factor, ADF, Surface-Associated Sulphydryl Protein, SASP, TXN, TRDX, TRX, TRX1
Accession:P10599
Sequence:Met 1-Val105
Fusion tag:N-6His
Activity:Measured by its ability to catalyze the reduction of insulin. The reaction leads to precipitation; which can be measured by absorbance at 650 nm. The specific activity is 0.2-1 Abs/min/mg; as measured under the described conditions.
Endotoxin:<1.0 EU per µg as determined by the LAL method.
Protein Construction:Recombinant Human Thioredoxin is produced by our E.coli expression system and the target gene encoding Met1-Val105 is expressed with a 6His tag at the N-terminus.
Purity:> 95 % as determined by reducing SDS-PAGE.
Mol Mass:13.9 kDa
AP Mol Mass:14 kDa
Formulation:Lyophilized from a 0.2 µm filtered solution of 20mM PB;150mM NaCl;PH7.2.
Shipping:This product is provided as lyophilized powder which is shipped with ice packs.
Stability and Storage:Lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Research Area:
Cell Biology
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