Human THAP1 Recombinant Protein (RPPB4947)
- SKU:
- RPPB4947
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NVV9
Description
| Product Name: | Human THAP1 Recombinant Protein |
| Product Code: | RPPB4947 |
| Size: | 20µg |
| Species: | Human |
| Target: | THAP1 |
| Synonyms: | THAP domain-containing protein 1, THAP1, DYT6. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | THAP1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 2M Urea, 10% glycerol, 1mM DTT and 0.2M NaCl. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMVQSCS AYGCKNRYDK DKPVSFHKFP LTRPSLCKEW EAAVRRKNFK PTKYSSICSE HFTPDCFKRE CNNKLLKENA VPTIFLCTEP HDKKEDLLEP QEQLPPPPLP PPVSQVDAAI GLLMPPLQTP VNLSVFCDHN YTVEDTMHQR KRIHQLEQQV EKLRKKLKTA QQRCRRQERQ LEKLKEVVHF QKEKDDVSER GYVILPNDYF EIVEVPA |
THAP domain-containing protein 1 (THAP1) is a 213 amino acid protein which is restricted to the nucleoplasm and contains one THAPtype zinc finger, a conserved DNA-binding domain. THAP1 is a DNA-binding transcription regulator which regulates endothelial cell proliferation and G1/S cell-cycle progression. In addition, THAP1 has a pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. THAP1 protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and serves as a proapoptotic factor which connects PAWR to PML nuclear bodies.
THAP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 237 amino acids (1-213 a.a) and having a molecular mass of 27.5kDa.THAP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | THAP1: DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. Specifically binds the 5'-[AT]NTNN[GT]GGCA[AGT]-3' core DNA sequence and acts by modulating expression of pRB-E2F cell-cycle target genes, including RRM1. Component of a THAP1/THAP3-HCFC1-OGT complex that is required for the regulation of the transcriptional activity of RRM1. May also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Defects in THAP1 are the cause of dystonia type 6 (DYT6). DYT6 is a primary torsion dystonia. Dystonia is defined by the presence of sustained involuntary muscle contractions, often leading to abnormal postures. Dystonia type 6 is characterized by onset in early adulthood, cranial or cervical involvement in about half of the cases, and frequent progression to involve multiple body regions. Belongs to the THAP1 family. |
| UniProt Protein Details: | Protein type:DNA-binding; Cell cycle regulation; Transcription factor Chromosomal Location of Human Ortholog: 8p11.21 Cellular Component: PML body; nucleus Molecular Function:protein binding; zinc ion binding; sequence-specific DNA binding Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; endothelial cell proliferation; regulation of mitotic cell cycle; cell cycle Disease: Dystonia 6, Torsion |
| NCBI Summary: | The protein encoded by this gene contains a THAP domain, a conserved DNA-binding domain. This protein colocalizes with the apoptosis response protein PAWR/PAR-4 in promyelocytic leukemia (PML) nuclear bodies, and functions as a proapoptotic factor that links PAWR to PML nuclear bodies. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q9NVV9 |
| NCBI GenInfo Identifier: | 29839656 |
| NCBI Gene ID: | 55145 |
| NCBI Accession: | Q9NVV9.1 |
| UniProt Secondary Accession: | Q9NVV9,Q53FQ1, Q6IA99, A6NCB6, D3DSY5, H9KV49, |
| UniProt Related Accession: | Q9NVV9 |
| Molecular Weight: | 213 |
| NCBI Full Name: | THAP domain-containing protein 1 |
| NCBI Synonym Full Names: | THAP domain containing, apoptosis associated protein 1 |
| NCBI Official Symbol: | THAP1�� |
| NCBI Official Synonym Symbols: | DYT6�� |
| NCBI Protein Information: | THAP domain-containing protein 1; 4833431A01Rik; THAP domain protein 1; nuclear proapoptotic factor |
| UniProt Protein Name: | THAP domain-containing protein 1 |
| Protein Family: | THAP domain-containing protein |
| UniProt Gene Name: | THAP1�� |
| UniProt Entry Name: | THAP1_HUMAN |
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