Human TGF beta Pathway Phosphorylation Array (8 targets) (SARB0069)

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SKU:
SARB0069
Product Type:
Phosphorylation Array
Analytes:
c-Jun (P-Ser73)
Analytes:
SMAD1 (P-Ser463/465)
Analytes:
SMAD5 (P-Ser463/465)
Analytes:
SMAD4 (P-Thr277)
Analytes:
ATF2 (P-Thr69/71)
Analytes:
C-Fos (P-Thr232)
Analytes:
SMAD2 (P-Ser245/250/255)
Analytes:
TAK1 (P-Ser412)
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0069
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:8
Gene Symbols:ATF2, FOS, JUN, MAP3K7, SMAD1, SMAD2, SMAD4, SMAD5
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:PKC Signaling / TGF-beta Signaling
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
c-Jun (P-Ser73)
  • Gene Symbol: JUN
  • Gene ID: 3725;
  • Accession #: P05412
SMAD1 (P-Ser463/465)
  • Gene Symbol: SMAD1 BSP1 MADH1 MADR1
  • Gene ID: 4086;
  • Accession #: Q15797
SMAD5 (P-Ser463/465)
  • Gene Symbol: SMAD5 MADH5
  • Gene ID: 4090;
  • Accession #: Q99717
SMAD4 (P-Thr277)
  • Gene Symbol: SMAD4 DPC4 MADH4
  • Gene ID: 4089;
  • Accession #: Q13485
ATF2 (P-Thr69/71)
  • Gene Symbol: ATF2 CREB2 CREBP1
  • Gene ID: 1386;
  • Accession #: P15336
C-Fos (P-Thr232)
  • Gene Symbol: FOS G0S7
  • Gene ID: 2353;
  • Accession #: P01100
SMAD2 (P-Ser245/250/255)
  • Gene Symbol: SMAD2 MADH2 MADR2
  • Gene ID: 4087;
  • Accession #: Q15796
TAK1 (P-Ser412)
  • Gene Symbol: MAP3K7 TAK1
  • Gene ID: 6885;
  • Accession #: O43318
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human TGF beta Phosphorylation Array C1 Membranes
  • Blocking Buffer
  • Detection Antibody Cocktail
  • 500X HRP-Anti-Rabbit IgG Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • 100x Phosphatase Inhibitor Cocktail I
  • Phosphatase Inhibitor Cocktail II
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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