Human TGF beta Array (25 targets) (SARB0070)

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SKU:
SARB0070
Product Type:
Protein Array
Analytes:
Activin A
Analytes:
Activin R1A (ALK-2)
Analytes:
BAMBI (NMA)
Analytes:
beta IG-H3
Analytes:
BMP-2
Analytes:
BMP-4
Analytes:
BMP-6
Analytes:
BMP-7
Analytes:
BMP-8
Analytes:
BMP-9
Analytes:
BMP-15 (GDF-9B)
Analytes:
BMPR-IA (ALK-3)
Analytes:
BMPR-II
Analytes:
Follistatin
Analytes:
GDF-11 (BMP-11)
Analytes:
GDF-15
Analytes:
Lefty-A
Analytes:
Noggin
Analytes:
SMAD3
Analytes:
SMAD4
Analytes:
TGF alpha
Analytes:
TGF beta 1
Analytes:
TGF beta 2
Analytes:
TGF-beta RII
Analytes:
TGF beta R3
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0070
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:25
Gene Symbols:ACVR1, BAMBI, BMP15, BMP2, BMP4, BMP6, BMP7, BMP8A, BMPR1A, BMPR2|FST, GDF11, GDF15, GDF2, INHBA, LEFTY2, MADH3, NOG, SMAD4, TGFA, TGFB1, TGFB2, TGFBI, TGFBR2, TGFBR3
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Activin A
  • Gene Symbol: INHBA
  • Gene ID: 3624
  • Accession #: P08476
Activin R1A (ALK-2)
  • Gene Symbol: ACVR1 ACVRLK2
  • Gene ID: 90
  • Accession #: Q04771
BAMBI (NMA)
  • Gene Symbol: BAMBI NMA
  • Gene ID: 25805
  • Accession #: Q13145
beta IG-H3
  • Gene Symbol: TGFBI BIGH3
  • Gene ID: 7045
  • Accession #: Q15582
BMP-2
  • Gene Symbol: BMP2 BMP2A
  • Gene ID: 650
  • Accession #: P12643
BMP-4
  • Gene Symbol: BMP4 BMP2B DVR4
  • Gene ID: 652
  • Accession #: P12644
BMP-6
  • Gene Symbol: BMP6 VGR
  • Gene ID: 654
  • Accession #: P22004
BMP-7
  • Gene Symbol: BMP7 OP1
  • Gene ID: 655
  • Accession #: P18075
BMP-8
  • Gene Symbol: BMP8A
  • Gene ID: 353500
  • Accession #: Q7Z5Y6
BMP-9
  • Gene Symbol: GDF2 BMP9
  • Gene ID: 2658
  • Accession #: Q9UK05
BMP-15 (GDF-9B)
  • Gene Symbol: BMP15 GDF9B
  • Gene ID: 9210
  • Accession #: O95972
BMPR-IA (ALK-3)
  • Gene Symbol: BMPR1A ACVRLK3 ALK3
  • Gene ID: 657
  • Accession #: P36894
BMPR-II
  • Gene Symbol: BMPR2 PPH1
  • Gene ID: 659
  • Accession #: Q13873
Follistatin
  • Gene Symbol: FST
  • Gene ID: 10468
  • Accession #: P19883
GDF-11 (BMP-11)
  • Gene Symbol: GDF11 BMP11
  • Gene ID: 10220
  • Accession #: O95390
GDF-15
  • Gene Symbol: GDF15 MIC1 PDF PLAB PTGFB
  • Gene ID: 9518
  • Accession #: Q99988
Lefty-A
  • Gene Symbol: LEFTY2 EBAF LEFTA LEFTYA TGFB4 PSEC0024
  • Gene ID: 7044
  • Accession #: O00292
Noggin
  • Gene Symbol: NOG
  • Gene ID: 9241
  • Accession #: Q13253
SMAD3
  • Gene Symbol: MADH3 SMAD3
  • Gene ID: 4088
  • Accession #: P84022
SMAD4
  • Gene Symbol: SMAD4 DPC4 MADH4
  • Gene ID: 4089
  • Accession #: Q13485
TGF alpha
  • Gene Symbol: TGFA
  • Gene ID: 7039
  • Accession #: P01135
TGF beta 1
  • Gene Symbol: TGFB1 TGFB
  • Gene ID: 7040
  • Accession #: P01137
TGF beta 2
  • Gene Symbol: TGFB2
  • Gene ID: 7042
  • Accession #: P61812
TGF-beta RII
  • Gene Symbol: TGFBR2
  • Gene ID: N/A
  • Accession #: P37173
TGF beta R3
  • Gene Symbol: TGFBR3
  • Gene ID: 7049
  • Accession #: Q03167
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human TGF beta Array C2 Membranes
  • Blocking Buffer
  • Biotinylated Antibody Cocktail
  • 1,000X HRP-Streptavidin Concentrate
  • 20X Wash Buffer I Concentrate
  • 20X Wash Buffer II Concentrate
  • 2X Cell Lysis Buffer Concentrate
  • Detection Buffer C
  • Detection Buffer D
  • 8-Well Incubation Tray w/ Lid
  • Protease Inhibitor Cocktail
  • Plastic Sheets
  • Array Map Template

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Detection Antibody Cocktail
4.Incubate with HRP-Conjugated anti-IgG
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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