Human TGF-beta 1 PharmaGenie ELISA Kit (SBRS1141)
- SKU:
- SBRS1141
Description
system_update_altDatasheet
Human TGF-beta 1 PharmaGenie ELISA Kit (SBRS1141)
| Product SKU: | SBRS1141 |
| Size: | 96T |
| Application: | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human ANG, CD23, Eotaxin, GCSF, GM-CSF, GRO-alpha, GRO-beta, GRO-gamma, I-309, IFN-gamma, IL-1 alpha, IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-10, IL-12 (p40), IL-12 (p70), IL-15, IL-16, IP-10, MCP-1, MCP-2, MCP-3, MCP-4, MCSF, MIG, MIP-1alpha, MIP-1beta, NAP-2, PDGF, PF-4, PARC, SCF, SDF-1alpha, TIMP-1, TIMP-2, TNF-alha, TGF-beta 2, TGF-beta 3, VEGF. |
| Uniprot: | P01137 |
| Gene ID: | 7040 |
| Gene Names: | TGFB / TGFB1 |
| Synonyms: | Transforming growth factor beta-1 (TGF-beta-1) [Cleaved into: Latency-associated peptide (LAP)] |
| Target Species: | Human |
| Compatible Sample Types: | Cell Culture Supernatants, Plasma, Serum |
| Design Principle: | Sandwich-based |
| Method of Detection: | Colorimetric |
| Quantitative/Semi-Quantitative: | Quantitative |
| Range: | 18 pg/ml - 4000 pg/ml |
| Sensitivity: | 18 pg/ml |
| Recommended Dilution: | 20 fold after treatment (see activation steps on page 6) |
| Storage/Stability: | The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C. |
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other materials and equipment required:
The Assay Genie Human TGF-beta 1 PharmaGenie ELISA Kit (SBRS1141) will require other equipment and materials to carry out the assay. Please see list below for further details.
- Distilled or deionized water
- Precision pipettes to deliver 2 ul to 1 ul volumes
- Adjustable 1-25 ul pipettes for reagent preparation
- 100 ul and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 ul of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 ul of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 ul of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 ul of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 ul of Stop Solution to each well.
- Read at 450 nm immediately.
| Bartuzi et al. | View Citation
Additional Information
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