Cytokines Recombinant Proteins
Human TFF3 Recombinant Protein (RPPB0964)
- SKU:
- RPPB0964
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q07654
- Research Area:
- Cytokines
Description
| Product Name: | Human TFF3 Recombinant Protein |
| Product Code: | RPPB0964 |
| Size: | 20µg |
| Species: | Human |
| Target: | TFF3 |
| Synonyms: | TFF-3, ITF, TFI, HITF, hP1.B, TFF3, Trefoil factor 3, Intestinal trefoil factor. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation: | Lyophilized from a 0.2�m filtered concentrated solution in PBS, pH 7.4. |
| Solubility: | It is recommended to reconstitute the lyophilized TFF3 in sterile 18M-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions. |
| Stability: | Lyophilized TFF3 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TFF3 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Purity: | Greater than 97.0% as determined by RP-HPLC and SDS-PAGE analysis. |
| Amino Acid Sequence: | EEYVGLSANQ CAVPAKDRVD CGYPHVTPKE CNNRGCCFDS RIPGVPWCFK�PLQEAECTF |
| Biological Activity: | The ED50 as determined by a chemotaxis bioassay using human MCF-7 cells is less than 10�g/ml, corresponding to a specific activity of > 100IU/mg. |
Proteins of the TFF family are characterized by obtaining a minimum of 1 copy of the trefoil motif, a 40-amino acid domain that contains 3 conserved disulfides. Trefoil Factors are stable secretory proteins expressed in gastrointestinal mucosa which protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium.TFF2 inhibits gastric acid motility & secretion. TFF2 stabilizes glycoproteins in the mucus gel through interactions with carbohydrate side chains. TFF3 induces ciliogenesis and promotes airway epithelial ciliated cell differentiation, relatively through an epidermal growth factor receptor-dependent pathway. TFF3 overexpression is crucial for progression in mouse and human hepatocellular carcinogenesis. TFF-3 is normally expressed in hepatocellular carcinoma and its expression associates with tumor grade.
TFF-3 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, polypeptide chain containing 2 x 59 amino acid chains which includes a 40 amino acid trefoil motif containing 3 conserved interamolecular disulfide bonds and having a total molecular mass of 13.2kDa. TFF-3 Human Recombinant is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TFF3: Involved in the maintenance and repair of the intestinal mucosa. Promotes the mobility of epithelial cells in healing processes (motogen). |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 21q22.3 Cellular Component: proteinaceous extracellular matrix; extracellular region; secretory granule Biological Process: response to peptide hormone stimulus; digestion; defense response |
| NCBI Summary: | Members of the trefoil family are characterized by having at least one copy of the trefoil motif, a 40-amino acid domain that contains three conserved disulfides. They are stable secretory proteins expressed in gastrointestinal mucosa. Their functions are not defined, but they may protect the mucosa from insults, stabilize the mucus layer and affect healing of the epithelium. This gene is expressed in goblet cells of the intestines and colon. This gene and two other related trefoil family member genes are found in a cluster on chromosome 21. [provided by RefSeq, Jul 2008] |
| UniProt Code: | Q07654 |
| NCBI GenInfo Identifier: | 585328 |
| NCBI Gene ID: | 7033 |
| NCBI Accession: | Q07654.1 |
| UniProt Secondary Accession: | Q07654,Q96NX0, Q9UDA5, E9PBB5, |
| UniProt Related Accession: | Q07654 |
| Molecular Weight: | 80 |
| NCBI Full Name: | Trefoil factor 3 |
| NCBI Synonym Full Names: | trefoil factor 3 (intestinal) |
| NCBI Official Symbol: | TFF3�� |
| NCBI Official Synonym Symbols: | ITF; P1B; TFI�� |
| NCBI Protein Information: | trefoil factor 3; polypeptide P1.B |
| UniProt Protein Name: | Trefoil factor 3 |
| UniProt Synonym Protein Names: | Intestinal trefoil factor; hITF; Polypeptide P1.B; hP1.B |
| Protein Family: | Trefoil factor |
| UniProt Gene Name: | TFF3�� |
| UniProt Entry Name: | TFF3_HUMAN |
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