Chemokines Recombinant Proteins

Human TECK Recombinant Protein (RPPB1264)

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RPPB1264

Description

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Product Name:Human TECK Recombinant Protein
Product Code:RPPB1264
Size:20µg
Species:Human
Target:TECK
Synonyms:C-C motif chemokine 25, Small-inducible cytokine A25, Thymus-expressed chemokine, Chemokine TECK, CCL25, SCYA25, TECK, Ckb15, MGC150327.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered White lyophilized (freeze-dried) powder.
Formulation:Filtered (0.2�m) and lyophilized from a concentrated (1mg/ml) solution in 20mM PB, pH 7.4, 150mM NaCl.
Solubility:It is recommended to reconstitute the lyophilized TECK in sterile 18M?-cm H2O not less than 100�g/ml, which can then be further diluted to other aqueous solutions.
Stability:Lyophilized TECK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution TECK should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.
Purity:Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Amino Acid Sequence:QGVFEDCCLA YHYPIGWAVL RRAWTYRIQE VSGSCNLPAA IFYLPKRHRK VCGNPKSREV QRAMKLLDAR NKVFAKLHHN MQTFQAGPHA VKKLSSGNSK LSSSKFSNPI SSSRKNVSLL ISANSGL
Biological Activity:Determined by its ability to chemoattract human monocytes using a concentration range of 1-10ng/ml corresponding to a Specific Activity of 100,000-1,000,000IU/mg.

CCL25 (Teck) is a novel CC chemokine, which is distantly related (about 20% amino acid sequence identity) to other CC chemokines. The mouse CCL25 cDNA has also been cloned and shown to encode a 144 a.a. protein, which exhibits 49% a.a. sequence identity to the human CCL25. Human and mouse CCL25 expression was shown to be greatly restricted to the thymus and small intestine. While dendritic cells are identified as the source of CCL25 production in the thymus, dendritic cells derived from bone marrow do not express CCL25. CCL25 signals through the CCR9 receptor. Teck is possibly involved in T-cell development. Recombinant human and mouse Teck were shown to be chemotactic for activated macrophages, dendritic cells and thymocytes. The recombinant protein demonstrates chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritic cells but is inactive on peripheral blood lymphocytes and neutrophils.

TECK Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 127 amino acids and having a molecular mass of 14.2kDa. The TECK is purified by proprietary chromatographic techniques.

UniProt Protein Function:CCL25: Potentially involved in T-cell development. Recombinant protein shows chemotactic activity on thymocytes, macrophages, THP-1 cells, and dendritics cells but is inactive on peripheral blood lymphocytes and neutrophils. Binds to CCR9. Isoform 2 is an antagonist of isoform 1. Belongs to the intercrine beta (chemokine CC) family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Chemokine; Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: extracellular space; extracellular region

Molecular Function:chemokine activity; chemokine receptor binding; CCR10 chemokine receptor binding; hormone activity

Biological Process: G-protein coupled receptor protein signaling pathway; cell surface receptor linked signal transduction; immune response; inflammatory response; chemotaxis; positive regulation of cell-matrix adhesion; leukocyte migration

NCBI Summary:This antimicrobial gene belongs to the subfamily of small cytokine CC genes. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for dendritic cells, thymocytes, and activated macrophages but is inactive on peripheral blood lymphocytes and neutrophils. The product of this gene binds to chemokine receptor CCR9. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2014]
UniProt Code:O15444
NCBI GenInfo Identifier:212286066
NCBI Gene ID:6370
NCBI Accession:O15444.2
UniProt Secondary Accession:O15444,Q96KJ7, A1L4J4, A6NI52, A8K9E7, B5MCA5,
UniProt Related Accession:O15444
Molecular Weight:16,538 Da
NCBI Full Name:C-C motif chemokine 25
NCBI Synonym Full Names:chemokine (C-C motif) ligand 25
NCBI Official Symbol:CCL25��
NCBI Official Synonym Symbols:TECK; Ckb15; SCYA25��
NCBI Protein Information:C-C motif chemokine 25; TECKvar; Ck beta-15; chemokine TECK; thymus expressed chemokine; thymus-expressed chemokine; small-inducible cytokine A25; small inducible cytokine subfamily A (Cys-Cys), member 25
UniProt Protein Name:C-C motif chemokine 25
UniProt Synonym Protein Names:Chemokine TECK; Small-inducible cytokine A25; Thymus-expressed chemokine
UniProt Gene Name:CCL25��
UniProt Entry Name:CCL25_HUMAN
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Additional Information

Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O15444
Research Area:
Chemokines
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