Enzymes Recombinant Proteins
Human TDP1 Recombinant Protein (RPPB2310)
- SKU:
- RPPB2310
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q9NUW8
- Research Area:
- Enzymes
Description
| Product Name: | Human TDP1 Recombinant Protein |
| Product Code: | RPPB2310 |
| Size: | 20µg |
| Species: | Human |
| Target: | TDP1 |
| Synonyms: | Tyrosyl-DNA phosphodiesterase 1, TDP1 protein, TDP1. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The TDP1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 0.4M urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MSQEGDYGRW TISSSDESEE EKPKPDKPST SSLLCARQGA ANEPRYTCSE AQKAAHKRKI SPVKFSNTDS VLPPKRQKSG SQEDLGWCLS SSDDELQPEM PQKQAEKVVI KKEKDISAPN DGTAQRTENH GAPACHRLKE EEDEYETSGE GQDIWDMLDK GNPFQFYLTR VSGVKPKYNS GALHIKDILS PLFGTLVSSA QFNYCFDVDW LVKQYPPEFR KKPILLVHGD KREAKAHLHA QAKPYENISL CQAKLDIAFG THHTKMMLLL YEEGLRVVIH TSNLIHADWH QKTQGTHL |
Tyrosyl-DNA phosphodiesterase 1 ( TDP1) is required for repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. TDP1 also detach glycolate from single-stranded DNA having 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks.
TDP1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 318 amino acids (1-298) and having a molecular mass of 35.8kDa. TDP1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | TDP1: DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 3'-phosphodiester bond, giving rise to DNA with a free 3' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase I active site tyrosine residue. Hydrolyzes 3'-phosphoglycolates on protruding 3' ends on DNA double-strand breaks due to DNA damage by radiation and free radicals. Acts on blunt-ended double-strand DNA breaks and on single-stranded DNA. Has low 3'exonuclease activity and can remove a single nucleoside from the 3'end of DNA and RNA molecules with 3'hydroxyl groups. Has no exonuclease activity towards DNA or RNA with a 3'phosphate. Monomer. Ubiquitously expressed. Similar expression throughout the central nervous system (whole brain, amygdala, caudate nucleus, cerebellum, cerebral cortex, frontal lobe, hippocampus, medulla oblongata, occipital lobe, putamen, substantia nigra, temporal lobe, thalamus, nucleus accumbens and spinal cord) and increased expression in testis and thymus. Belongs to the tyrosyl-DNA phosphodiesterase family. |
| UniProt Protein Details: | Protein type:Phosphodiesterase; EC 3.1.4.-; DNA repair, damage Chromosomal Location of Human Ortholog: 14q32.11 Cellular Component: cytoplasm; nucleus Molecular Function:tyrosyl-DNA phosphodiesterase activity; protein binding; double-stranded DNA binding; exonuclease activity; single-stranded DNA binding Biological Process: single strand break repair; double-strand break repair; DNA repair Disease: Spinocerebellar Ataxia, Autosomal Recessive, With Axonal Neuropathy |
| NCBI Summary: | The protein encoded by this gene is involved in repairing stalled topoisomerase I-DNA complexes by catalyzing the hydrolysis of the phosphodiester bond between the tyrosine residue of topoisomerase I and the 3-prime phosphate of DNA. This protein may also remove glycolate from single-stranded DNA containing 3-prime phosphoglycolate, suggesting a role in repair of free-radical mediated DNA double-strand breaks. This gene is a member of the phospholipase D family and contains two PLD phosphodiesterase domains. Mutations in this gene are associated with the disease spinocerebellar ataxia with axonal neuropathy (SCAN1). [provided by RefSeq, Aug 2016] |
| UniProt Code: | Q9NUW8 |
| NCBI GenInfo Identifier: | 37999797 |
| NCBI Gene ID: | 55775 |
| NCBI Accession: | Q9NUW8.2 |
| UniProt Related Accession: | Q9NUW8 |
| Molecular Weight: | Predicted Molecular Mass: 20.4kDaAccurate Molecular Mass: 25kDa |
| NCBI Full Name: | Tyrosyl-DNA phosphodiesterase 1 |
| NCBI Synonym Full Names: | tyrosyl-DNA phosphodiesterase 1 |
| NCBI Official Symbol: | TDP1�� |
| NCBI Protein Information: | tyrosyl-DNA phosphodiesterase 1 |
| UniProt Protein Name: | Tyrosyl-DNA phosphodiesterase 1 |
| Protein Family: | Tyrosyl-DNA phosphodiesterase |
| UniProt Gene Name: | TDP1�� |
| UniProt Entry Name: | TYDP1_HUMAN |
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