Human TCP1 Recombinant Protein (RPPB4930)
- SKU:
- RPPB4930
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P17987
Description
| Product Name: | Human TCP1 Recombinant Protein |
| Product Code: | RPPB4930 |
| Size: | 10µg |
| Species: | Human |
| Target: | TCP1 |
| Synonyms: | T-complex protein 1 subunit alpha, TCP-1-alpha, CCT-alpha, TCP1, CCT1, CCTA, D6S230E. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The TCP1 solution (0.5 mg/ml) contains 20mM Tris-HCl Buffer (pH 8.0), 1mM DTT, 0.1mM PMSF and 10% Glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks.�Store, frozen at -20°C for longer periods of time.�For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).�Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 80.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MEGPLSVFGD RSTGETIRSQ NVMAAASIAN IVKSSLGPVG LDKMLVDDIG DVTITNDGAT ILKLLEVEHP AAKVLCELAD LQDKEVGDGT TSVVIIAAEL LKNADELVKQ KIHPTSVISG YRLACKEAVR YINENLIVNT DELGRDCLIN AAKTSMSSKI IGINGDFFAN MVVDAVLAIK YTDIRGQPRY PVNSVNILKA HGRSQMESML ISGYALNCVV GSQGMPKRIV NAKIACLDFS LQKTKMKLGV QVVITDPEKL DQIRQRESDI TKERIQKILA TGANVILTTG GIDDMCLKYF VEAGAMAVRR VLKRDLKRIA KASGATILST LANLEGEETF EAAMLGQAEE VVQERICDDE LILIKNTKAR TSASIILRGA NDFMCDEMER SLHDALCVVK RVLESKSVVP GGGAVEAALS IYLENYATSM GSREQLAIAE FARSLLVIPN TLAVNAAQDS TDLVAKLRAF HNEAQVNPER KNLKWIGLDL SNGKPRDNKQ AGVFEPTIVK VKSLKFATEA AITILRIDDL IKLHPESKDD KHGSYEDAVH SGALND |
TCP1 is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of 2 identical stacked rings, each containing eight different proteins. Unfolded polypeptides penetrate the central cavity of the complex and are folded in an ATP-dependent manner. The TCP1 protein is found in the cytosol as a subunit of a hetero-oligomeric chaperone. TCP1 has a significant function in maintaining cellular homoeostasis by assisting the folding of many proteins such as the cytoskeletal components actin and tubulin.
TCP1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 576 amino acids (1-556 a.a.) and having a molecular mass of 62.5kDa. The TCP1 is purified by proprietary chromatographic techniques.
| UniProt Protein Function: | CCT-alpha: Molecular chaperone; assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin. Heterooligomeric complex of about 850 to 900 kDa that forms two stacked rings, 12 to 16 nm in diameter. Interacts with PACRG. Component of the BBS/CCT complex composed at least of MKKS, BBS10, BBS12, TCP1, CCT2, CCT3, CCT4, CCT5 AND CCT8. Belongs to the TCP-1 chaperonin family. |
| UniProt Protein Details: | Protein type:Chaperone; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 6q25.3-q26 Cellular Component: chaperonin-containing T-complex; Golgi apparatus; centrosome; microtubule; pericentriolar material; acrosome; nuclear heterochromatin; cytosol Molecular Function:protein binding; ubiquitin protein ligase binding; unfolded protein binding; ATP binding Biological Process: tubulin folding; 'de novo' posttranslational protein folding; cellular protein metabolic process; protein folding; binding of sperm to zona pellucida |
| NCBI Summary: | The protein encoded by this gene is a molecular chaperone that is a member of the chaperonin containing TCP1 complex (CCT), also known as the TCP1 ring complex (TRiC). This complex consists of two identical stacked rings, each containing eight different proteins. Unfolded polypeptides enter the central cavity of the complex and are folded in an ATP-dependent manner. The complex folds various proteins, including actin and tubulin. Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized. In addition, three pseudogenes that appear to be derived from this gene have been found. [provided by RefSeq, Jun 2010] |
| UniProt Code: | P17987 |
| NCBI GenInfo Identifier: | 135538 |
| NCBI Gene ID: | 6950 |
| NCBI Accession: | P17987.1 |
| UniProt Secondary Accession: | P17987,Q15556, Q5TCM3, E1P5B2, |
| UniProt Related Accession: | P17987 |
| Molecular Weight: | 556 |
| NCBI Full Name: | T-complex protein 1 subunit alpha |
| NCBI Synonym Full Names: | t-complex 1 |
| NCBI Official Symbol: | TCP1�� |
| NCBI Official Synonym Symbols: | CCT1; CCTa; D6S230E; CCT-alpha; TCP-1-alpha�� |
| NCBI Protein Information: | T-complex protein 1 subunit alpha; tailless complex polypeptide 1; T-complex protein 1, alpha subunit |
| UniProt Protein Name: | T-complex protein 1 subunit alpha |
| UniProt Synonym Protein Names: | CCT-alpha |
| Protein Family: | T-complex protein |
| UniProt Gene Name: | TCP1�� |
| UniProt Entry Name: | TCPA_HUMAN |
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