Human TCEA1 Recombinant Protein (RPPB4920)

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SKU:
RPPB4920
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
P23193

Description

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Product Name:Human TCEA1 Recombinant Protein
Product Code:RPPB4920
Size:20µg
Species:Human
Target:TCEA1
Synonyms:Transcription elongation factor A (SII) 1, Transcription elongation factor TFIIS.o, TCEA, GTF2S, SII, TF2S.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:The TCEA1 solution (1mg/1ml) contains 20mM Tris-HCl buffer (pH 7.5), 1mM DTT, 50mM NaCl and 20% glycerol.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMEDEVV RFAKKMDKMV QKKNAAGALD LLKELKNIPM TLELLQSTRI GMSVNAIRKQ STDEEVTSLA KSLIKSWKKL LDGPSTEKDL DEKKKEPAIT SQNSPEAREE STSSGNVSNR KDETNARDTY VSSFPRAPST SDSVRLKCRE MLAAALRTGD DYIAIGADEE ELGSQIEEAI YQEIRNTDMK YKNRVRSRIS NLKDAKNPNL RKNVLCGNIP PDLFARMTAE EMASDELKEM RKNLTKEAIR EHQMAKTGGT QTDLFTCGKC KKKNCTYTQV QTRSADEPMT TFVVCNECGN RWKFC

TCEA1 is a member of the TFS-II family. TCEA1 binds to Pol II and operates to cleave the emerging transcript, thus unlocking the complex and permitting transcription to continue. TCEA1 is localized to the nucleus and holds three independently-folding domains that are essential for accurate binding to Pol II. The arresting sites in are able to trap a certain segment of elongating RNA polymerases that goes through, causing in a locked ternary complex. Cleavage of the emerging transcript by S-II enables the renewal of elongation from the new 3'-terminus.

TCEA1 Human Recombinant produced in E. coli is a single polypeptide chain containing 325 amino acids (1-301) and having a molecular mass of 36.5kDa.TCEA1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:TFIIS: Necessary for efficient RNA polymerase II transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary complexes. Cleavage of the nascent transcript by S-II allows the resumption of elongation from the new 3'-terminus. A chromosomal aberration involving TCEA1 may be a cause of salivary gland pleiomorphic adenomas (PA) [181030]. Pleiomorphic adenomas are the most common benign epithelial tumors of the salivary gland. Translocation t(3;8)(p21;q12) with PLAG1. Belongs to the TFS-II family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 8q11.23

Cellular Component: nucleolus; nucleoplasm; nucleus; transcription factor TFIID complex

Molecular Function:protein binding

Biological Process: RNA elongation from RNA polymerase II promoter; transcription from RNA polymerase II promoter; transcription-coupled nucleotide-excision repair

UniProt Code:P23193
NCBI GenInfo Identifier:1174652
NCBI Gene ID:6917
NCBI Accession:P23193.2
UniProt Secondary Accession:P23193,Q15563, Q6FG87, A6NF25, A8K339,
UniProt Related Accession:P23193
Molecular Weight:34kDa
NCBI Full Name:Transcription elongation factor A protein 1
NCBI Synonym Full Names:transcription elongation factor A1
NCBI Official Symbol:TCEA1��
NCBI Official Synonym Symbols:SII; TCEA; TF2S; GTF2S; TFIIS��
NCBI Protein Information:transcription elongation factor A protein 1
UniProt Protein Name:Transcription elongation factor A protein 1
UniProt Synonym Protein Names:Transcription elongation factor S-II protein 1; Transcription elongation factor TFIIS.o
Protein Family:Tuliposide A-converting enzyme
UniProt Gene Name:TCEA1��
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