ADAM17 is a member of the ADAM (a disintegrin and metalloproteinase domain) family. Members of this family have been linked to a variety of biologic processes that include cell-cell and cell-matrix interactions, such as fertilization, muscle development, and neurogenesis. Inflammatory skin and bowel disease are just two diseases linked to ADAM17. The Assay Genie TACE/ADAM17 ELISA is a highly sensitive assay for the quantitative measurement of TACE/ADAM17 in serum, blood, plasma, cell culture supernatant and tissue samples.
This immunoassay kit allows for the in vitro quantitative determination of Human ADAM17 concentrations in serum plasma and other biological fluids.
Sensitivity:
37.5pg/ml
Range:
62.5-4000pg/ml
Storage:
4°C for 6 months
Note:
For Research Use Only
Recovery:
Matrices listed below were spiked with certain level of Human ADAM17 and the recovery rates were calculated by comparing the measured value to the expected amount of Human ADAM17 in samples.
Matrix
Recovery range(%)
Average(%)
serum(n=5)
86-105
99
EDTA plasma(n=5)
86-102
95
UFH plasma(n=5)
86-102
94
Linearity:
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of Human ADAM17 and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
Sample
1:2
1:4
1:8
serum(n=5)
86-92%
85-103%
88-98%
EDTA plasma(n=5)
85-100%
84-94%
82-98%
UFH plasma(n=5)
80-96%
81-97%
80-90%
CV(%):
Intra-Assay: CV<8% Inter-Assay: CV<10%
Component
Quantity
Storage
ELISA Microplate (Dismountable)
8×12 strips
4°C for 6 months
Lyophilized Standard
2
4°C/-20°C
Sample/Standard Dilution Buffer
20ml
4°C
Biotin-labeled Antibody(Concentrated)
120ul
4°C (Protect from light)
Antibody Dilution Buffer
10ml
4°C
HRP-Streptavidin Conjugate(SABC)
120ul
4°C (Protect from light)
SABC Dilution Buffer
10ml
4°C
TMB Substrate
10ml
4°C (Protect from light)
Stop Solution
10ml
4°C
Wash Buffer(25X)
30ml
4°C
Plate Sealer
5
-
Other materials and equipment required:
Microplate reader with 450 nm wavelength filter
Multichannel Pipette, Pipette, microcentrifuge tubes and disposable pipette tips
TACE: a type I membrane protein with disintegrin and metalloprotease activity. An ubiquitously expressed ectoenzyme of peptidase family M12B. Must be membrane anchored to cleave the different substrates. The cytoplasmic domain is not required for the this activity. Possesses a narrow endopeptidase specificity. Cleaves Pro-Leu-Ala-Gln-Ala--Val-Arg-Ser-Ser-Ser in the membrane-bound, 26-kDa form of tumor necrosis factor alpha (TNF-alpha) to its mature soluble form. Responsible for the proteolytic release of several other cell-surface proteins, including p75 TNF-receptor, interleukin 1 recept
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