Human T Cell Response Array (10 targets) (SARB0067)

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SKU:
SARB0067
Product Type:
Protein Array
Analytes:
IL-2
Analytes:
IL-4
Analytes:
IL-5
Analytes:
IL-9
Analytes:
IL-10
Analytes:
IL-12 p70
Analytes:
IL-17A
Analytes:
IFN-gamma
Analytes:
TGF beta 1
Analytes:
TNF alpha
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0067
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:10
Gene Symbols:IFNG, IL10, IL12A, IL17A, IL2, IL4, IL5, IL9, TGFB1, TNF
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker/ Key Factor Screening; Identifying Key Factors; Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
IL-2
  • Gene Symbol: IL2 IL-2
  • Gene ID: 3558
  • Accession #: P60568
IL-4
  • Gene Symbol: IL4
  • Gene ID: 3565
  • Accession #: P05112
IL-5
  • Gene Symbol: IL5
  • Gene ID: 3567
  • Accession #: P05113
IL-9
  • Gene Symbol: IL9
  • Gene ID: 3578
  • Accession #: P15248
IL-10
  • Gene Symbol: IL10
  • Gene ID: 3586
  • Accession #: P22301
IL-12 p70
  • Gene Symbol: IL12A NKSF1
  • Gene ID: 3592
  • Accession #: P29459
IL-17A
  • Gene Symbol: IL17A
  • Gene ID: 3605
  • Accession #: Q16552
IFN-gamma
  • Gene Symbol: IFNG
  • Gene ID: 3458
  • Accession #: P01579
TGF beta 1
  • Gene Symbol: TGFB1 TGFB
  • Gene ID: 7040
  • Accession #: P01137
TNF alpha
  • Gene Symbol: TNF TNFA TNFSF2
  • Gene ID: 7124
  • Accession #: P01375
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human T Cell Response Array C1 Membranes
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated HRP
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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