Human SYT5 Recombinant Protein (RPPB4890)

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SKU:
RPPB4890
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
O00445

Description

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Product Name:Human SYT5 Recombinant Protein
Product Code:RPPB4890
Size:10µg
Species:Human
Target:SYT5
Synonyms:Synaptotagmin V, Synaptotagmin 5, synaptotagmin-5, sytV, SytV.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:SYT5 protein solution (0.25mg/ml) containing 20mMTris-HCl(pH 8.0) containing 40% glycerol, 0.2M NaCl and 2mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 90.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSLGRLQYS LDYDFQSGQL LVGILQAMGL AALDLGGSSD PYVRVYLLPDKRRRYETKVH RQTLNPHFGE TFAFKVPYVE LGGRVLVMAV YDFDRFSRND AIGEVRVPMS SVDLGRPVQAWRELQAAPRE EQEKLGDICF SLRYVPTAGK LTVIVLEAKN LKKMDVGGLS DPYVKVHLLQ GGKKVRKKKT TIKKNTLNPY YNEAFSFEVPCDQVQKVQVE LTVLDYDKLG KNEAIGRVAV GAAAGGAGLR HWADMLANPR RPIAQWHSLR PPDRVRLLPA P

SynaptotagminV, (SYT5) is a member of synaptotagmin family, which is a family of type IIImembrane proteins characterized by cytoplasmic repeats related to proteinkinase C regulatory (C2) domains that are considered to bind calcium.Synaptotagmins function as negative regulators of vesicle fusion, allowingfusion in the attendance of calcium, and as calcium receptors or sensormolecules. Among the diseases associated with SYT5 are labyrinthitis, andthyroiditis.

SYT5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 301 amino acids (109-386aa) and having a molecular mass of 33.6kDa.SYT5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:SYT5: May be involved in Ca(2+)-dependent exocytosis of secretory vesicles through Ca(2+) and phospholipid binding to the C2 domain or may serve as Ca(2+) sensors in the process of vesicular trafficking and exocytosis. Regulates the Ca(2+)- dependent secretion of norepinephrine in PC12 cells. Required for export from the endocytic recycling compartment to the cell surface. Belongs to the synaptotagmin family.
UniProt Protein Details:

Protein type:Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.42|11p

Cellular Component: plasma membrane; synaptic vesicle membrane

Molecular Function:calcium ion binding; calcium-dependent phospholipid binding; clathrin binding; syntaxin binding

Biological Process: calcium ion-dependent exocytosis of neurotransmitter; regulation of calcium ion-dependent exocytosis; synaptic transmission; synaptic vesicle endocytosis; vesicle fusion

NCBI Summary:Synaptotagmins, such as SYT5, are a family of type III membrane proteins characterized by cytoplasmic repeats related to protein kinase C (see MIM 176960) regulatory (C2) domains, which are thought to bind calcium. Synaptotagmins may act both as negative regulators of vesicle fusion, allowing fusion in the presence of calcium, and as calcium receptors or sensor molecules (summary by Hudson and Birnbaum, 1995 [PubMed 7597049]).[supplied by OMIM, Feb 2011]
UniProt Code:O00445
NCBI GenInfo Identifier:32700066
NCBI Gene ID:6861
NCBI Accession:O00445.2
UniProt Secondary Accession:O00445,Q86X72, B3KWJ8, B7Z300,
UniProt Related Accession:O00445
Molecular Weight:42,474 Da
NCBI Full Name:Synaptotagmin-5
NCBI Synonym Full Names:synaptotagmin 5
NCBI Official Symbol:SYT5��
NCBI Protein Information:synaptotagmin-5
UniProt Protein Name:Synaptotagmin-5
UniProt Synonym Protein Names:Synaptotagmin V; SytV
Protein Family:Synaptotagmin
UniProt Gene Name:SYT5��
UniProt Entry Name:SYT5_HUMAN
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