Human STRADA Recombinant Protein (RPPB4855)
- SKU:
- RPPB4855
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- Q7RTN6
Description
| Product Name: | Human STRADA Recombinant Protein |
| Product Code: | RPPB4855 |
| Size: | 25µg |
| Species: | Human |
| Target: | STRADA |
| Synonyms: | LYK5, NY-BR-96, PMSE, Stlk, STRAD, STE20-related kinase adapter protein alpha, STRAD alpha, STE20-related adapter protein, Serologically defined breast cancer antigen NY-BR-96, STRADA. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | STRADA protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH 8.0), 0.4M Urea and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMSFLVSK PERIRTNDAS SESIASFSKQ EVMSSFLPEG GCYELLTVIG KGFEDLMTVN LARYKPTGEY VTVRRINLEA CSNEMVTFLQ GELHVSKLFN HPNIVPYRAT FIADNELWVV TSFMAYGSAK DLICTHFMDG MNELAIAYIL QGVLKALDYI HHMGYVHRSV KASHILISVD GKVYLSGLRS NLSMISHGQR QRVVHDFPKY SVKVLPWLSP EVLQQNLQGY DAKSDIYSVG ITACELANGH VPFKDMPATQ MLLEKLNGTV PCLLDTSTIP AEELTMSPSR SVANSGLSDS LTTSTPRPSN GPVPAPS |
STE20-Related Kinase Adaptor Alpha (STRADA) is a part of the STE- 20 like kinase family which stimulates MAPK pathways by activating MAPKKK. STRADA is an upstream activator of LKB1 and also leads the sub-cellular localization of LKB1 by fixing it in the cytoplasm. The interaction between STRADA-LKB1 leads to phosphorylation of STRADA and enhanced autophosphorylation of LKB1.
STRADA Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 337 amino acids (1-314a.a) and having a molecular mass of 37kDa. STRADA is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | STRAD: a pseudokinase which, in complex with CAB39, binds to and activates LKB1. Relocates LKB1 from the nucleus to the cytoplasm. Plays an essential role in LKB1-mediated G1 cell cycle arrest. 3 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Kinase, protein; Motility/polarity/chemotaxis; Protein kinase, STE; Protein kinase, Ser/Thr (non-receptor); STE group; STE20 family; STLK subfamily Chromosomal Location of Human Ortholog: 17q23.3 Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus Molecular Function:kinase binding; protein binding; protein kinase activator activity; protein kinase activity; protein serine/threonine kinase activator activity Biological Process: activation of protein kinase activity; cell cycle arrest; protein amino acid phosphorylation; protein export from nucleus Disease: Polyhydramnios, Megalencephaly, And Symptomatic Epilepsy |
| NCBI Summary: | The protein encoded by this gene contains a STE20-like kinase domain, but lacks several residues that are critical for catalytic activity, so it is termed a 'pseudokinase'. The protein forms a heterotrimeric complex with serine/threonine kinase 11 (STK11, also known as LKB1) and the scaffolding protein calcium binding protein 39 (CAB39, also known as MO25). The protein activates STK11 leading to the phosphorylation of both proteins and excluding STK11 from the nucleus. The protein is necessary for STK11-induced G1 cell cycle arrest. A mutation in this gene has been shown to result in polyhydramnios, megalencephaly, and symptomatic epilepsy (PMSE) syndrome. Multiple transcript variants encoding different isoforms have been found for this gene. Additional transcript variants have been described but their full-length nature is not known. [provided by RefSeq, Sep 2009] |
| UniProt Code: | Q7RTN6 |
| NCBI GenInfo Identifier: | 74759034 |
| NCBI Gene ID: | 92335 |
| NCBI Accession: | Q7RTN6.1 |
| UniProt Secondary Accession: | Q7RTN6,Q5JPI2, Q7Z4K9, Q8NC31, Q8NCF1, Q9H272, B4DDE3 B4DW17, J3KTC9, |
| UniProt Related Accession: | Q7RTN6 |
| Molecular Weight: | 34,625 Da |
| NCBI Full Name: | STE20-related kinase adapter protein alpha |
| NCBI Synonym Full Names: | STE20-related kinase adaptor alpha |
| NCBI Official Symbol: | STRADA�� |
| NCBI Official Synonym Symbols: | LYK5; PMSE; Stlk; STRAD; NY-BR-96�� |
| NCBI Protein Information: | STE20-related kinase adapter protein alpha |
| UniProt Protein Name: | STE20-related kinase adapter protein alpha |
| UniProt Synonym Protein Names: | STE20-related adapter protein; Serologically defined breast cancer antigen NY-BR-96 |
| UniProt Gene Name: | STRADA�� |
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