Human Stem Cell Array (15 targets) (SARB0066)

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SKU:
SARB0066
Product Type:
Protein Array
Analytes:
Alpha-fetoprotein
Analytes:
BMPR-IA (ALK-3)
Analytes:
ALK-6 (BMPR-IB)
Analytes:
Brachyury (Protein T)
Analytes:
CD38
Analytes:
E-Cadherin
Analytes:
GATA4
Analytes:
hCG beta
Analytes:
Nanog
Analytes:
Nestin
Analytes:
OCT-4
Analytes:
PDX-1 (IPF1)
Analytes:
SOX17
Analytes:
SOX2
Analytes:
VEGFR2
Reactivity:
Human
Applications:
Multiplex Array

Description

system_update_altDatasheet
Product Sku:SARB0066
Size:2, 4, or 8
Species Detected:Human
Number of Targets Detected:15
Gene Symbols:AFP, BMPR1A, BMPR1B, CD38, CDH1, CGB, GATA4, KDR, NANOG, NES|PDX1, POU5F1, SOX17, SOX2, T
Compatible Sample Types:Cell Culture Supernatants, Cell Lysates, Plasma, Serum, Tissue Lysates
Design Principle:Sandwich-based
Method of Detection:Chemiluminescence
Pathway:
Quantitative/Semi-Quantitative:Semi-Quantitative
Solid Support:Membrane
Suggested Application(s):Multiplexed Protein Detection Detection of Relative Protein Expression Detecting Patterns of Cytokine Expression Biomarker/ Key Factor Screening Identifying Key Factors Confirming a Biological Process
Storage/Stability:For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period.
Once thawed, store array membranes and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
Hover over target for more information
Alpha-fetoprotein
  • Gene Symbol: AFP HPAFP
  • Gene ID: 174
  • Accession #: P02771
BMPR-IA (ALK-3)
  • Gene Symbol: BMPR1A ACVRLK3 ALK3
  • Gene ID: 657
  • Accession #: P36894
ALK-6 (BMPR-IB)
  • Gene Symbol: BMPR1B
  • Gene ID: 658
  • Accession #: O00238
Brachyury (Protein T)
  • Gene Symbol: T
  • Gene ID: 6862
  • Accession #: O15178
CD38
  • Gene Symbol: CD38
  • Gene ID: 952
  • Accession #: P28907
E-Cadherin
  • Gene Symbol: CDH1 CDHE UVO
  • Gene ID: 999
  • Accession #: P12830
GATA4
  • Gene Symbol: GATA4
  • Gene ID: 2626
  • Accession #: P43694
hCG beta
  • Gene Symbol: CGB CGB3; CGB5; CGB7; CGB8
  • Gene ID: 1082; 93659; 94027; 94115
  • Accession #: P01233
Nanog
  • Gene Symbol: NANOG
  • Gene ID: 79923
  • Accession #: Q9H9S0
Nestin
  • Gene Symbol: NES Nbla00170
  • Gene ID: 10763
  • Accession #: P48681
OCT-4
  • Gene Symbol: POU5F1 OCT3 OCT4 OTF3
  • Gene ID: 5460
  • Accession #: Q01860
PDX-1 (IPF1)
  • Gene Symbol: PDX1 IPF1 STF1
  • Gene ID: 3651
  • Accession #: P52945
SOX17
  • Gene Symbol: SOX17
  • Gene ID: 64321
  • Accession #: Q9H6I2
SOX2
  • Gene Symbol: SOX2
  • Gene ID: 6657
  • Accession #: P48431
VEGFR2
  • Gene Symbol: KDR FLK1 VEGFR2
  • Gene ID: 3791
  • Accession #: P35968
  • Easy to use
  • No specialized equipment needed
  • Compatible with nearly any liquid sample
  • Proven technology
  • Highly sensitive (pg/ml)
  • Sandwich ELISA specificity
  • Higher density than ELISA, Western blot or bead-based multiplex
  • Human Stem Cell Array C1 Membranes(s)
  • Blocking Buffer
  • Wash Buffer 1
  • Wash Buffer 2
  • Biotinylated Detection Antibody Cocktail
  • Streptavidin-Conjugated Fluor
  • Detection Buffer C
  • Detection Buffer D
  • Lysis Buffer
  • 8-Well Incubation Tray
  • Plastic Sheets
  • Array Templates

Other Materials Required

  • Pipettors, pipet tips and other common lab consumables
  • Orbital shaker or oscillating rocker
  • Tissue Paper, blotting paper or chromatography paper
  • Adhesive tape or Saran Wrap
  • Distilled or de-ionized water
  • A chemiluminescent blot documentation system , X-ray Film and a suitable film processor, or another chemiluminescent detection system.
1.Block membranes
2.Incubate with Sample
3.Incubate with Biotinylated Detection Antibody Cocktail
4.Incubate with HRP-Conjugated Streptavidin
5.Incubate with Detection Buffers
6.Image with chemiluminescent imaging system
7.Perform densitometry and analysis
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