Human STAP1 Recombinant Protein (RPPB4840)

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SKU:
RPPB4840
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9ULZ2

Description

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Product Name:Human STAP1 Recombinant Protein
Product Code:RPPB4840
Size:20µg
Species:Human
Target:STAP1
Synonyms:Signal-transducing adaptor protein 1, STAP-1, BCR downstream-signaling protein 1, Docking protein BRDG1, Stem cell adaptor protein 1, STAP1, BRDG1.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered colorless solution.
Formulation:STAP1 protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 30% glycerol, 0.1M NaCl and 1mM DTT.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.
Purity:Greater than 95.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSHMMAKKP PKPAPRRIFQ ERLKITALPL YFEGFLLIKR SGYREYEHYW TELRGTTLFF YTDKKSIIYV DKLDIVDLTC LTEQNSTEKN CAKFTLVLPK EEVQLKTENT ESGEEWRGFI LTVTELSVPQ NVSLLPGQVI KLHEVLEREK KRRIETEQST SVEKEKEPTE DYVDVLNPMP ACFYTVSRKE ATEMLQKNPS LGNMILRPGS DSRNYSITIR QEIDIPRIKH YKVMSVGQNY TIELEKPVTL PNLFSVIDYF VKETRGNLRP FICSTDENTG QEPSMEGRSE KLKKNPHIA

Signal-transducing adaptor protein 1 (STAP1) acts as a docking protein which operates downstream of Tec tyrosine kinase in B cell antigen receptor signaling. The STAP1 protein is directly phosphorylated by Tec in vitro where it partakes in a positive feedback loop, increasing Tec activity.

STAP1 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 319 amino acids (1-295 a.a) and having a molecular mass of 36.8kDa.STAP1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:STAP1: an adaptor protein which contains pleckstrin homology (PH) and Src homology 2-like (SRC) domains and a proline-rich region. Induced in activated bone marrow-derived macrophages, and by CpG, LPS, and interferon in myeloid cell lines. Induces pro-inflammatory responses and may contribute to neuronal apoptosis and degeneration. Overexpressed, it interacts with the CSFR, inhibiting its ligand-dependent phosphorylation. Its expression inhibits cellular migration and can increase cytotoxicity against 661W photoreceptor like cells.Protein type: Adaptor/scaffoldChromosomal Location of Human Ortholog: 4q13.2Cellular Component: cytoplasm; mitochondrion; nucleus; protein complexMolecular Function: phosphotyrosine binding; protein binding; protein kinase binding; SH3/SH2 adaptor activity; transmembrane receptor protein tyrosine kinase adaptor protein activityBiological Process: negative regulation of phosphorylation; positive regulation of B cell receptor signaling pathway; positive regulation of phagocytosis, engulfment; transmembrane receptor protein tyrosine kinase signaling pathway
UniProt Protein Details:
NCBI Summary:The protein encoded by this gene contains a proline-rich region, a pleckstrin homology (PH) domain, and a region in the carboxy terminal half with similarity to the Src Homology 2 (SH2) domain. This protein is a substrate of tyrosine-protein kinase Tec, and its interaction with tyrosine-protein kinase Tec is phosphorylation-dependent. This protein is thought to participate in a positive feedback loop by upregulating the activity of tyrosine-protein kinase Tec. Variants of this gene have been associated with autosomal-dominant hypercholesterolemia (ADH), which is characterized by elevated low-density lipoprotein cholesterol levels and in increased risk of coronary vascular disease. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]
UniProt Code:Q9ULZ2
NCBI GenInfo Identifier:62511239
NCBI Gene ID:26228
NCBI Accession:Q9ULZ2.1
UniProt Secondary Accession:Q9ULZ2,B2R980
UniProt Related Accession:Q9ULZ2
Molecular Weight:34,291 Da
NCBI Full Name:Signal-transducing adaptor protein 1
NCBI Synonym Full Names:signal transducing adaptor family member 1
NCBI Official Symbol:STAP1��
NCBI Official Synonym Symbols:BRDG1; STAP-1��
NCBI Protein Information:signal-transducing adaptor protein 1
UniProt Protein Name:Signal-transducing adaptor protein 1
UniProt Synonym Protein Names:BCR downstream-signaling protein 1; Docking protein BRDG1; Stem cell adaptor protein 1
Protein Family:Signal-transducing adaptor protein
UniProt Gene Name:STAP1��
UniProt Entry Name:STAP1_HUMAN
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