Enzymes Recombinant Proteins

Human ST3GAL5 Recombinant Protein (RPPB2286)

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SKU:
RPPB2286
Product Type:
Recombinant Protein
Species:
Human
Uniprot:
Q9UNP4
Research Area:
Enzymes

Description

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Product Name:Human ST3GAL5 Recombinant Protein
Product Code:RPPB2286
Size:20µg
Species:Human
Target:ST3GAL5
Synonyms:ST3 Beta-Galactoside Alpha-2,3-Sialyltransferase 5, SIAT9,Sialyltransferase 9 (CMP-NeuAc:Lactosylceramide Alpha-2,3-Sialyltransferase;GM3 Synthase), Ganglioside GM3 Synthase, ST3GalV, CMP-NeuAc:LactosylceramideAlpha-2,3-Sialyltransferase, ST3Gal V, EC 2.4.99.9, SATI, SIATGM3S, Alpha2,3-Sialyltransferase V, Lactosylceramide Alpha-2,3-Sialyltransferase, Sialyltransferase9, ST3GALV, GM3 Synthase.
Source:Escherichia Coli
Physical Appearance:Sterile Filtered clear solution.
Formulation:ST3GAL5 protein solution (1mg/ml) containing In 20mM Tris-HCl buffer (pH 8.0), 10% glyceroland 0.4M Urea.
Stability:Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please avoid freeze thaw cycles.
Purity:Greaterthan 85.0% as determined by SDS-PAGE.
Amino Acid Sequence:MGSSHHHHHH SSGLVPRGSH MGSLKLNYTT EECDMKKMHY VDPDHVKRAQ KYAQQVLQKE CRPKFAKTSMALLFEHRYSV DLLPFVQKAP KDSEAESKYD PPFGFRKFSS KVQTLLELLP EHDLPEHLKA KTCRRCVVIGSGGILHGLEL GHTLNQFDVV IRLNSAPVEG YSEHVGNKTT IRMTYPEGAP LSDLEYYSND LFVAVLFKSV DFNWLQAMVK KETLPFWVRLFFWKQVAEKI PLQPKHFRIL NPVIIKETAF DILQYSEPQS RFWGRDKNVP TIGVIAVVLA THLCDEVSLAGFGYDLNQPR TPLHYFDSQC MAAMNFQTMH NVTTETKFLL KLVKEGVVKD LSGGIDREF

ST3Beta-Galactoside Alpha-2,3-Sialyltransferase 5, also know as ST3GAL5 belongs tothe glycosyltransferase family 29 and localized to the Golgi apparatus. ST3GAL5is known to play a part in the induction of cell differentiation, modulation ofcell proliferation,maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. ST3GAL5 is atype II membrane protein which catalyzes the formation of GM3 with lactosylceramideas the substrate. Mutation in this ST3GAL5 has been connected with Amishinfantile epilepsy syndrome. Transcript variants encoding various isoforms havebeen found for this gene.

ST3GAL5 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 359 amino acids (83-418a.a) and having a molecular mass of 41kDa. ST3GAL5 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

UniProt Protein Function:ST3GAL5: Catalyzes the formation of ganglioside GM3 (alpha-N- acetylneuraminyl-2,3-beta-D-galactosyl-1, 4-beta-D- glucosylceramide). Defects in ST3GAL5 are the cause of Amish infantile epilepsy syndrome (AIES). AIES is an autosomal recessive, infantile-onset symptomatic epilepsy associated with developmental stagnation and blindness. Belongs to the glycosyltransferase 29 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Membrane protein, integral; Glycan Metabolism - glycosphingolipid biosynthesis - ganglio series; EC 2.4.99.9; Transferase

Chromosomal Location of Human Ortholog: 2p11.2

Cellular Component: Golgi membrane; integral to plasma membrane; integral to membrane; integral to Golgi membrane

Molecular Function:lactosylceramide alpha-2,3-sialyltransferase activity; neolactotetraosylceramide alpha-2,3-sialyltransferase activity; sialyltransferase activity

Biological Process: cellular protein metabolic process; glycosphingolipid biosynthetic process; dolichol-linked oligosaccharide biosynthetic process; carbohydrate metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; ganglioside biosynthetic process

Disease: Amish Infantile Epilepsy Syndrome

NCBI Summary:Ganglioside GM3 is known to participate in the induction of cell differentiation, modulation of cell proliferation, maintenance of fibroblast morphology, signal transduction, and integrin-mediated cell adhesion. The protein encoded by this gene is a type II membrane protein which catalyzes the formation of GM3 using lactosylceramide as the substrate. The encoded protein is a member of glycosyltransferase family 29 and may be localized to the Golgi apparatus. Mutation in this gene has been associated with Amish infantile epilepsy syndrome. Transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:Q9UNP4
NCBI GenInfo Identifier:109633044
NCBI Gene ID:8869
NCBI Accession:NP_003887.3
UniProt Secondary Accession:Q9UNP4,O94902, Q53QU1, Q6NZX4, Q6YFL1, B3KM82, D6W5L9
UniProt Related Accession:Q9UNP4
Molecular Weight:45,585 Da
NCBI Full Name:lactosylceramide alpha-2,3-sialyltransferase isoform 1
NCBI Synonym Full Names:ST3 beta-galactoside alpha-2,3-sialyltransferase 5
NCBI Official Symbol:ST3GAL5��
NCBI Official Synonym Symbols:SATI; SIAT9; ST3GalV; SIATGM3S��
NCBI Protein Information:lactosylceramide alpha-2,3-sialyltransferase
UniProt Protein Name:Lactosylceramide alpha-2,3-sialyltransferase
UniProt Synonym Protein Names:CMP-NeuAc:lactosylceramide alpha-2,3-sialyltransferase; Ganglioside GM3 synthase; ST3Gal V; ST3GalV; Sialyltransferase 9
Protein Family:Lactosylceramide alpha-2,3-sialyltransferase
UniProt Gene Name:ST3GAL5��
UniProt Entry Name:SIAT9_HUMAN
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