Description
| Product Name: | Human SRGN Recombinant Protein |
| Product Code: | RPPB4823 |
| Size: | 20µg |
| Species: | Human |
| Target: | SRGN |
| Synonyms: | Serglycin, PRG, PRG1, PPG, Proteoglycan 1 secretory granule, Hematopoetic proteoglycan core protein, Platelet proteoglycan core protein, proteoglycan protein core for mast cell secretory granule. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered colorless solution. |
| Formulation: | The SRGN solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 2mM DTT, 0.15M NaCl and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 90% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSHMYPTRR ARYQWVRCNP DSNSANCLEE KGPMFELLPG ESNKIPRLRT DLFPKTRIQD LNRIFPLSED YSGSGFGSGS GSGSGSGSGF LTEMEQDYQL VDESDAFHDN LRSLDRNLPS DSQDLGQHGL EEDFML |
SRGN is identified as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of various hematopoietic cells also hold a protease-resistant peptide core, and is vital for neutralizing hydrolytic enzymes. SRGN is related to the macromolecular complex of granzymes and perforin that acts as a intermediary of granule-mediated apoptosis.
SRGN Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 156 amino acids (28-158) and having a molecular mass of 17.4 kDa (Molecular weight on SDS-PAGE will appear higher).SRGN is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | SRGN: Plays a role in formation of mast cell secretory granules and mediates storage of various compounds in secretory vesicles. Required for storage of some proteases in both connective tissue and mucosal mast cells and for storage of granzyme B in T-lymphocytes. Plays a role in localizing neutrophil elastase in azurophil granules of neutrophils. Mediates processing of MMP2. Plays a role in cytotoxic cell granule-mediated apoptosis by forming a complex with granzyme B which is delivered to cells by perforin to induce apoptosis. Regulates the secretion of TNF- alpha and may also regulate protease secretion. Inhibits bone mineralization. Belongs to the serglycin family. |
| UniProt Protein Details: | Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 10q22.1 Cellular Component: Golgi membrane; Golgi apparatus; extracellular space; mast cell granule; extracellular region Molecular Function:collagen binding; protein binding Biological Process: maintenance of granzyme B localization in T cell secretory granule; platelet activation; negative regulation of bone mineralization; platelet degranulation; induction of apoptosis by granzyme; biomineral formation; negative regulation of cytokine secretion; T cell secretory granule organization and biogenesis; maintenance of protease localization in mast cell secretory granule; protein processing; mast cell secretory granule organization and biogenesis; blood coagulation |
| NCBI Summary: | This gene encodes a protein best known as a hematopoietic cell granule proteoglycan. Proteoglycans stored in the secretory granules of many hematopoietic cells also contain a protease-resistant peptide core, which may be important for neutralizing hydrolytic enzymes. This encoded protein was found to be associated with the macromolecular complex of granzymes and perforin, which may serve as a mediator of granule-mediated apoptosis. Two transcript variants, only one of them protein-coding, have been found for this gene. [provided by RefSeq, Jul 2010] |
| UniProt Code: | P10124 |
| NCBI GenInfo Identifier: | 269849659 |
| NCBI Gene ID: | 5552 |
| NCBI Accession: | P10124.3 |
| UniProt Secondary Accession: | P10124,Q5VW06, B2R4L7, |
| UniProt Related Accession: | P10124 |
| Molecular Weight: | 158 |
| NCBI Full Name: | Serglycin |
| NCBI Synonym Full Names: | serglycin |
| NCBI Official Symbol: | SRGN�� |
| NCBI Official Synonym Symbols: | PPG; PRG; PRG1�� |
| NCBI Protein Information: | serglycin; p.PG; serglycin proteoglycan; proteoglycan 1, secretory granule; platelet proteoglycan core protein; hematopoetic proteoglycan core peptide; hematopoetic proteoglycan core protein; hematopoietic proteoglycan core protein; secretory granule proteoglycan core peptide; secretory granule proteoglycan core protein; proteoglycan protein core for mast cell secretory granule |
| UniProt Protein Name: | Serglycin |
| UniProt Synonym Protein Names: | Hematopoietic proteoglycan core protein; Platelet proteoglycan core protein; P.PG; Secretory granule proteoglycan core protein |
| Protein Family: | Serglycin |
| UniProt Gene Name: | SRGN�� |
| UniProt Entry Name: | SRGN_HUMAN |
