Human SPI1 Recombinant Protein (RPPB4804)
- SKU:
- RPPB4804
- Product Type:
- Recombinant Protein
- Species:
- Human
- Uniprot:
- P17947
Description
| Product Name: | Human SPI1 Recombinant Protein |
| Product Code: | RPPB4804 |
| Size: | 10µg |
| Species: | Human |
| Target: | SPI1 |
| Synonyms: | Transcription factor PU.1, 31 kDa-transforming protein, Transcription factor PU.1 isoform 1, SPI1, OF, PU.1, SFPI1, SPI-1, SPI-A. |
| Source: | Escherichia Coli |
| Physical Appearance: | Sterile Filtered clear solution. |
| Formulation: | The SPI1 solution (0.25mg/1ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
| Stability: | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Purity: | Greater than 85.0% as determined by SDS-PAGE. |
| Amino Acid Sequence: | MGSSHHHHHH SSGLVPRGSH MGSMLQACKM EGFPLVPPQP SEDLVPYDTD LYQRQTHEYY PYLSSDGESH SDHYWDFHPH HVHSEFESFA ENNFTELQSV QPPQLQQLYR HMELEQMHVL DTPMVPPHPS LGHQVSYLPR MCLQYPSLSP AQPSSDEEEG ERQSPPLEVS DGEADGLEPG PGLLPGETGS KKKIRLYQFL LDLLRSGDMK DSIWWVDKDK GTFQFSSKHK EALAHRWGIQ KGNRKKMTYQ KMARALRNYG KTGEVKKVKK KLTYQFSGEV LGRGGLAERR HPPH |
Spi-1Proto-Oncogene, also known as SPI1, owns ETS-domain transcription factor whichactivates gene expression during myeloid and Blymphoid cell development. SPI1 bindsto a purine-rich sequence called the PU-box which is located near the promotersof target genes, and regulates their expression. SPI1 binds RNA and modulatepre-mRNA splicing. SPI1 is also regulates alternative splicing of targetgenes.
SPI1 Human Recombinant produced in E. coli is a single polypeptide chain containing 294 amino acids (1-271) and having a molecular mass of 33.6kDa.SPI1 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
| UniProt Protein Function: | PU.1: Binds to the PU-box, a purine-rich DNA sequence (5'- GAGGAA-3') that can act as a lymphoid-specific enhancer. This protein is a transcriptional activator that may be specifically involved in the differentiation or activation of macrophages or B- cells. Also binds RNA and may modulate pre-mRNA splicing. Binds DNA as a monomer. Interacts with CEBPD and NONO. Interacts with RUNX1 and SPIB. Interacts with GFI1; the interaction represses SPI1 transcriptional activity. Highly expressed in both FV-P and FV-A-induced erythro- leukemia cell lines that have undergone rearrangements of the Spi- 1 gene due to the insertion of SFFV. Belongs to the ETS family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:DNA-binding; Transcription factor; Oncoprotein Chromosomal Location of Human Ortholog: 11p11.2 Cellular Component: nuclear chromatin Molecular Function:NFAT protein binding; protein binding; RNA binding; RNA polymerase II transcription factor activity, enhancer binding; transcription factor activity Biological Process: anatomical structure regression; cell differentiation; erythrocyte differentiation; granulocyte differentiation; lymphocyte differentiation; lymphoid progenitor cell differentiation; macrophage differentiation; myeloid dendritic cell differentiation; negative regulation of gene expression, epigenetic; negative regulation of MHC class II biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent; regulation of erythrocyte differentiation; somatic stem cell maintenance; transcription from RNA polymerase II promoter |
| NCBI Summary: | This gene encodes an ETS-domain transcription factor that activates gene expression during myeloid and B-lymphoid cell development. The nuclear protein binds to a purine-rich sequence known as the PU-box found near the promoters of target genes, and regulates their expression in coordination with other transcription factors and cofactors. The protein can also regulate alternative splicing of target genes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P17947 |
| NCBI GenInfo Identifier: | 60415923 |
| NCBI Gene ID: | 6688 |
| NCBI Accession: | P17947.2 |
| UniProt Secondary Accession: | P17947,P17433, Q6BDS1, |
| UniProt Related Accession: | P17947 |
| Molecular Weight: | 31,211 Da |
| NCBI Full Name: | Transcription factor PU.1 |
| NCBI Synonym Full Names: | Spi-1 proto-oncogene |
| NCBI Official Symbol: | SPI1�� |
| NCBI Official Synonym Symbols: | OF; PU.1; SFPI1; SPI-1; SPI-A�� |
| NCBI Protein Information: | transcription factor PU.1 |
| UniProt Protein Name: | Transcription factor PU.1 |
| UniProt Synonym Protein Names: | 31 kDa-transforming protein |
| Protein Family: | Transcription factor |
| UniProt Gene Name: | SPI1�� |
| UniProt Entry Name: | SPI1_HUMAN |
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