Human SPARC (Osteonectin) PharmaGenie ELISA Kit (SBRS1114)

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SKU:
SBRS1114
Product Type:
ELISA Kit
Reactivity:
Human
Applications:
ELISA
ELISA Type:
Sandwich

Description

system_update_altDatasheet

Human SPARC (Osteonectin) PharmaGenie ELISA Kit (SBRS1114)

Product SKU:SBRS1114
Size:96T
Application:This ELISA antibody pair detects Human SPARC. Other species not determined.
Uniprot:P09486
Gene ID:6678
Gene Names:ON / SPARC
Synonyms:SPARC (Basement-membrane protein 40) (BM-40) (Osteonectin) (ON) (Secreted protein acidic and rich in cysteine)
Target Species:Human
Compatible Sample Types:Cell Culture Supernatants, Plasma, Serum
Design Principle:Sandwich-based
Method of Detection:Colorimetric
Quantitative/Semi-Quantitative:Quantitative
Range:0.11 ng/ml - 31.25 ng/ml
Sensitivity:0.11 ng/ml
Recommended Dilution:80 fold
Storage/Stability:The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
  1. Pre-Coated 96-well Strip Microplate
  2. Wash Buffer
  3. Stop Solution
  4. Assay Diluent(s)
  5. Lyophilized Standard
  6. Biotinylated Detection Antibody
  7. Streptavidin-Conjugated HRP
  8. TMB One-Step Substrate

Other materials and equipment required:

The Assay Genie Human SPARC (Osteonectin) PharmaGenie ELISA Kit (SBRS1114) will require other equipment and materials to carry out the assay. Please see list below for further details.

  • Distilled or deionized water
  • Precision pipettes to deliver 2 ul to 1 ul volumes
  • Adjustable 1-25 ul pipettes for reagent preparation
  • 100 ul and 1 liter graduated cylinders
  • Tubes to prepare standard and sample dilutions
  • Absorbent paper
  • Microplate reader capable of measuring absorbance at 450nm
  • Log-log graph paper or computer and software for ELISA data analysis
  1. Prepare all reagents, samples and standards as instructed in the manual.
  2. Add 100 ul of standard or sample to each well.
  3. Incubate 2.5 h at RT or O/N at 4°C.
  4. Add 100 ul of prepared biotin antibody to each well.
  5. Incubate 1 h at RT.
  6. Add 100 ul of prepared Streptavidin solution to each well.
  7. Incubate 45 min at RT.
  8. Add 100 ul of TMB One-Step Substrate Reagent to each well.
  9. Incubate 30 min at RT.
  10. Add 50 ul of Stop Solution to each well.
  11. Read at 450 nm immediately.
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